論文 - 詳細
| RRC ID | 76080 |
|---|---|
| 著者 | Velez-Aguilera G, Ossareh-Nazari B, Van Hove L, Joly N, Pintard L. |
| タイトル | Cortical microtubule pulling forces contribute to the union of the parental genomes in the Caenorhabditis elegans zygote. |
| ジャーナル | Elife |
| Abstract |
Previously, we reported that the Polo-like kinase PLK-1 phosphorylates the single Caenorhabditis elegans lamin (LMN-1) to trigger lamina depolymerization during mitosis. We showed that this event is required to form a pronuclear envelope scission event that removes membranes on the juxtaposed oocyte and sperm pronuclear envelopes in the zygote, allowing the parental chromosomes to merge in a single nucleus after segregation (Velez-Aguilera et al., 2020). Here, we show that cortical microtubule pulling forces contribute to pronuclear envelopes scission by promoting mitotic spindle elongation, and conversely, nuclear envelopes remodeling facilitates spindle elongation. We also demonstrate that weakening the pronuclear envelopes via PLK-1-mediated lamina depolymerization, is a prerequisite for the astral microtubule pulling forces to trigger pronuclear membranes scission. Finally, we provide evidence that PLK-1 mainly acts via lamina depolymerization in this process. These observations thus indicate that temporal coordination between lamina depolymerization and mitotic spindle elongation facilitates pronuclear envelopes scission and parental genomes unification. |
| 巻・号 | 11 |
| 公開日 | 2022-3-8 |
| DOI | 10.7554/eLife.75382 |
| PII | 75382 |
| PMID | 35259092 |
| PMC | PMC8956289 |
| MeSH | Animals Caenorhabditis elegans* / genetics Caenorhabditis elegans Proteins* / genetics Embryo, Nonmammalian Laminin / genetics Microtubules Mitosis Protein Serine-Threonine Kinases / genetics Spindle Apparatus Zygote |
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オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1502 |