論文 - 詳細
| RRC ID | 76083 |
|---|---|
| 著者 | Vieira AFC, Xatse MA, Tifeki H, Diot C, Walhout AJM, Olsen CP. |
| タイトル | Monomethyl branched-chain fatty acids are critical for Caenorhabitis elegans survival in elevated glucose conditions. |
| ジャーナル | J Biol Chem |
| Abstract |
The maintenance of optimal membrane composition under basal and stress conditions is critical for the survival of an organism. High-glucose stress has been shown to perturb membrane properties by decreasing membrane fluidity, and the membrane sensor PAQR-2 is required to restore membrane integrity. However, the mechanisms required to respond to elevated dietary glucose are not fully established. In this study, we used a 13C stable isotope-enriched diet and mass spectrometry to better understand the impact of glucose on fatty acid dynamics in the membrane of Caenorhabditis elegans. We found a novel role for monomethyl branched-chain fatty acids (mmBCFAs) in mediating the ability of the nematodes to survive conditions of elevated dietary glucose. This requirement of mmBCFAs is unique to glucose stress and was not observed when the nematode was fed elevated dietary saturated fatty acid. In addition, when worms deficient in elo-5, the major biosynthesis enzyme of mmBCFAs, were fed Bacillus subtilis (a bacteria strain rich in mmBCFAs) in combination with high glucose, their survival rates were rescued to wild-type levels. Finally, the results suggest that mmBCFAs are part of the PAQR-2 signaling response during glucose stress. Taken together, we have identified a novel role for mmBCFAs in stress response in nematodes and have established these fatty acids as critical for adapting to elevated glucose. |
| 巻・号 | 298(2) |
| ページ | 101444 |
| 公開日 | 2022-2-1 |
| DOI | 10.1016/j.jbc.2021.101444 |
| PII | S0021-9258(21)01253-9 |
| PMID | 34826420 |
| PMC | PMC8819037 |
| MeSH | Animals Caenorhabditis elegans* / metabolism Caenorhabditis elegans Proteins* / chemistry Fatty Acids* / metabolism Glucose* / metabolism Membrane Fluidity / physiology Membrane Proteins |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 12 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm3410 |