論文 - 詳細
| RRC ID | 76111 |
|---|---|
| 著者 | Almanzar DE, Hamrick A, Rog O. |
| タイトル | Single-sister labeling in the C. elegans germline using the nucleotide analog EdU. |
| ジャーナル | STAR Protoc |
| Abstract |
Reciprocal exchanges between genetically identical sister chromatids (sister chromatid exchanges or SCEs) have been challenging to study. Here, we describe a protocol that utilizes a pulse/chase of the thymidine analog 5-ethyl-3'-deoxyuridine (EdU) in combination with click chemistry and antibody labeling to selectively label sister chromatids in the C. elegans germline. Labeling has no discernable effects on meiosis, allowing for cytological quantification of SCEs. This protocol can be combined with a variety of imaging approaches, including STED, confocal and super-resolution. For complete details on the use and execution of this protocol, please refer to Almanzar et al. (2021). |
| 巻・号 | 3(2) |
| ページ | 101344 |
| 公開日 | 2022-6-17 |
| DOI | 10.1016/j.xpro.2022.101344 |
| PII | S2666-1667(22)00224-6 |
| PMID | 35509971 |
| PMC | PMC9059099 |
| MeSH | Animals Caenorhabditis elegans* / genetics Deoxyuridine / chemistry* Germ Cells Meiosis Nucleotides Sister Chromatid Exchange* |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm611 |