Reference - Detail
| RRC ID | 76131 |
|---|---|
| Author | Calvi I, Schwager F, Gotta M. |
| Title | PP1 phosphatases control PAR-2 localization and polarity establishment in C. elegans embryos. |
| Journal | J Cell Biol |
| Abstract |
Cell polarity relies on the asymmetric distribution of the conserved PAR proteins, which is regulated by phosphorylation/dephosphorylation reactions. While the kinases involved have been well studied, the role of phosphatases remains poorly understood. In Caenorhabditis elegans zygotes, phosphorylation of the posterior PAR-2 protein by the atypical protein kinase PKC-3 inhibits PAR-2 cortical localization. Polarity establishment depends on loading of PAR-2 at the posterior cortex. We show that the PP1 phosphatases GSP-1 and GSP-2 are required for polarity establishment in embryos. We find that codepletion of GSP-1 and GSP-2 abrogates the cortical localization of PAR-2 and that GSP-1 and GSP-2 interact with PAR-2 via a PP1 docking motif in PAR-2. Mutating this motif in vivo, to prevent binding of PAR-2 to PP1, abolishes cortical localization of PAR-2, while optimizing this motif extends PAR-2 cortical localization. Our data suggest a model in which GSP-1/-2 counteracts PKC-3 phosphorylation of PAR-2, allowing its cortical localization at the posterior and polarization of the one-cell embryo. |
| Volume | 221(10) |
| Published | 2022-10-3 |
| DOI | 10.1083/jcb.202201048 |
| PII | 213453 |
| PMID | 36083688 |
| PMC | PMC9467853 |
| MeSH | Animals Caenorhabditis elegans* / genetics Caenorhabditis elegans* / metabolism Caenorhabditis elegans Proteins* / genetics Caenorhabditis elegans Proteins* / metabolism Cell Polarity Embryo, Nonmammalian / metabolism Phosphoric Monoester Hydrolases* / metabolism Protein Kinase C / metabolism |
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 44 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| C.elegans | tm7819 |