Reference - Detail
| RRC ID | 76174 |
|---|---|
| Author | Godini R, Pocock R. |
| Title | Characterization of the Doublesex/MAB-3 transcription factor DMD-9 in Caenorhabditis elegans. |
| Journal | G3 (Bethesda) |
| Abstract |
DMD-9 is a Caenorhabditis elegans Doublesex/MAB-3 Domain transcription factor (TF) of unknown function. Single-cell transcriptomics has revealed that dmd-9 is highly expressed in specific head sensory neurons, with lower levels detected in non-neuronal tissues (uterine cells and sperm). Here, we characterized endogenous dmd-9 expression and function in hermaphrodites and males to identify potential sexually dimorphic roles. In addition, we dissected the trans- and cis-regulatory mechanisms that control DMD-9 expression in neurons. Our results show that of the 22 neuronal cell fate reporters we assessed in DMD-9-expressing neurons, only the neuropeptide-encoding flp-19 gene is cell-autonomously regulated by DMD-9. Further, we did not identify defects in behaviors mediated by DMD-9 expressing neurons in dmd-9 mutants. We found that dmd-9 expression in neurons is regulated by 4 neuronal fate regulatory TFs: ETS-5, EGL-13, CHE-1, and TTX-1. In conclusion, our study characterized the DMD-9 expression pattern and regulatory logic for its control. The lack of detectable phenotypes in dmd-9 mutant animals suggests that other proteins compensate for its loss. |
| Volume | 13(2) |
| Published | 2023-2-9 |
| DOI | 10.1093/g3journal/jkac305 |
| PII | 6858940 |
| PMID | 36454093 |
| PMC | PMC9911054 |
| MeSH | Animals Caenorhabditis elegans* / metabolism Caenorhabditis elegans Proteins* / metabolism DNA-Binding Proteins / metabolism Male Neurons / metabolism Semen / metabolism Transcription Factors / genetics Transcription Factors / metabolism |
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 5 |
| Altmetric score changes over past 6months | 0.2 |
| Resource | |
| C.elegans | tm866 tm4583 tm252 tm1734 tm242 |