RRC ID 76189
Author Hendi A, Niu LG, Snow AW, Ikegami R, Wang ZW, Mizumoto K.
Title Channel-independent function of UNC-9/Innexin in spatial arrangement of GABAergic synapses in C. elegans.
Journal Elife
Abstract Precise synaptic connection of neurons with their targets is essential for the proper functioning of the nervous system. A plethora of signaling pathways act in concert to mediate the precise spatial arrangement of synaptic connections. Here we show a novel role for a gap junction protein in controlling tiled synaptic arrangement in the GABAergic motor neurons in Caenorhabditis elegans, in which their axons and synapses overlap minimally with their neighboring neurons within the same class. We found that while EGL-20/Wnt controls axonal tiling, their presynaptic tiling is mediated by a gap junction protein UNC-9/Innexin, that is localized at the presynaptic tiling border between neighboring dorsal D-type GABAergic motor neurons. Strikingly, the gap junction channel activity of UNC-9 is dispensable for its function in controlling tiled presynaptic patterning. While gap junctions are crucial for the proper functioning of the nervous system as channels, our finding uncovered the novel channel-independent role of UNC-9 in synapse patterning.
Volume 11
Published 2022-11-15
DOI 10.7554/eLife.80555
PII 80555
PMID 36378164
PMC PMC9665852
MeSH Animals Caenorhabditis elegans* / physiology Caenorhabditis elegans Proteins* / genetics Caenorhabditis elegans Proteins* / metabolism Connexins / genetics Connexins / metabolism Membrane Proteins / genetics Membrane Proteins / metabolism Motor Neurons / metabolism Synapses / metabolism
C.elegans tm5479 tm4133