論文 - 詳細
| RRC ID | 76190 |
|---|---|
| 著者 | Heo W, Hwang H, Kim J, Oh SH, Yu Y, Lee JH, Kim K. |
| タイトル | The CCAAT-box transcription factor, NF-Y complex, mediates the specification of the IL1 neurons in C. elegans. |
| ジャーナル | BMB Rep |
| Abstract |
Neuronal differentiation is highly coordinated through a cascade of gene expression, mediated via interactions between transacting transcription factors and cis-regulatory elements of their target genes. However, the mechanisms of transcriptional regulation that determine neuronal cell-fate are not fully understood. Here, we show that the nuclear transcription factor Y (NF-Y) subunit, NFYA-1, is necessary and sufficient to express the flp-3 neuropeptide gene in the IL1 neurons of C. elegans. flp-3 expression is decreased in dorsal and lateral, but not ventral IL1s of nfya-1 mutants. The expression of another terminally differentiated gene, eat-4 vesicular glutamate transporter, is abolished, whereas the unc-8 DEG/ENaC gene and pan-neuronal genes are expressed normally in IL1s of nfya-1 mutants. nfya-1 is expressed in and acts in IL1s to regulate flp-3 and eat-4 expression. Ectopic expression of NFYA-1 drives the expression of flp-3 gene in other cell-types. Promoter analysis of IL1-expressed genes results in the identification of several cisregulatory motifs which are necessary for IL1 expression, including a putative CCAAT-box located in the flp-3 promoter that NFYA-1 directly interacts with. NFYA-1 and NFYA-2, together with NFYB-1 and NFYC-1, exhibit partly or fully redundant roles in the regulation of flp-3 or unc-8 expression, respectively. Taken together, our data indicate that the NF-Y complex regulates neuronal subtype-specification via regulating a set of terminal-differentiation genes. [BMB Reports 2023; 56(3): 153-159]. |
| 巻・号 | 56(2) |
| ページ | 153-159 |
| 公開日 | 2023-3-1 |
| DOI | 10.5483/BMBRep.2022-0146 |
| PII | 5722 |
| PMID | 36330709 |
| PMC | PMC10068339 |
| MeSH | Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins* / genetics Caenorhabditis elegans Proteins* / metabolism Gene Expression Regulation Ion Channels / metabolism Neurons / metabolism Promoter Regions, Genetic / genetics Transcription Factors* / genetics Transcription Factors* / metabolism |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Bluesky |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 1.2 |
| リソース情報 | |
| 線虫 | tm4194 tm4541 |