RRC ID 76227
Author Liu M, Li N, Shan S, Shi Y, Zhu Y, Lu W.
Title Acanthopanax senticosus Polysaccharide Enhances the Pathogen Resistance of Radiation-Damaged Caenorhabditis elegans through Intestinal p38 MAPK-SKN-1/ATF-7 Pathway and Stress Response.
Journal Int J Mol Sci
Abstract With the advancement of science and technology, humans are chronically exposed to ionizing radiation. It is crucial to look for efficient and low-toxic anti-radiation agents. Through preliminary screening, we found that Acanthopanax senticosus polysaccharide (ASPS) played a major role in regulating immune damage caused by radiation. The objective of this study was to apply the Caenorhabditis elegans-P. aeruginosa (PA14) infection model to illuminate the mechanism of ASPS increasing the pathogen resistance of radiation-damaged nematodes. Results indicated that ASPS (1 mg/mL) significantly enhanced the pathogen resistance of radiation-damaged nematodes by directly elevating the immune response of nematodes rather than by affecting the bacterial activity. Through further research on the p38 MAPK signaling pathway and related mutants, we found that ASPS functioned by the p38 MAPK pathway in the intestine, and SKN-1, ATF-7 as the downstream targets of PMK-1 participated the regulation of ASPS. In addition, ASPS markedly alleviated the stress status of damaged nematodes by regulating oxidative stress. Collectively, our findings suggest that ASPS enhances the pathogen resistance of radiation-damaged nematodes through the intestinal p38MAPK-SKN-1/ATF-7 pathway and stress response.
Volume 23(9)
Published 2022-5-1
DOI 10.3390/ijms23095034
PII ijms23095034
PMID 35563423
PMC PMC9103771
MeSH Activating Transcription Factors* / genetics Activating Transcription Factors* / metabolism Animals Caenorhabditis elegans / metabolism Caenorhabditis elegans / radiation effects Caenorhabditis elegans Proteins* / metabolism Caenorhabditis elegans Proteins* / radiation effects DNA-Binding Proteins / metabolism Eleutherococcus Immunity, Innate / genetics Intestines / metabolism Polysaccharides* / metabolism Polysaccharides* / pharmacology Stress, Physiological / genetics Transcription Factors* / genetics Transcription Factors* / metabolism p38 Mitogen-Activated Protein Kinases* / metabolism
Resource
C.elegans tm4392