| RRC ID |
76260
|
| 著者 |
Paschinger K, Wöls F, Yan S, Jin C, Vanbeselaere J, Dutkiewicz Z, Arcalis E, Malzl D, Wilson IBH.
|
| タイトル |
N-glycan antennal modifications are altered in Caenorhabditis elegans lacking the HEX-4 N-acetylgalactosamine-specific hexosaminidase.
|
| ジャーナル |
J Biol Chem
|
| Abstract |
Simple organisms are often considered to have simple glycomes, but plentiful paucimannosidic and oligomannosidic glycans overshadow the less abundant N-glycans with highly variable core and antennal modifications; Caenorhabditis elegans is no exception. By use of optimized fractionation and assessing wildtype in comparison to mutant strains lacking either the HEX-4 or HEX-5 β-N-acetylgalactosaminidases, we conclude that the model nematode has a total N-glycomic potential of 300 verified isomers. Three pools of glycans were analyzed for each strain: either PNGase F released and eluted from a reversed-phase C18 resin with either water or 15% methanol or PNGase Ar released. While the water-eluted fractions were dominated by typical paucimannosidic and oligomannosidic glycans and the PNGase Ar-released pools by glycans with various core modifications, the methanol-eluted fractions contained a huge range of phosphorylcholine-modified structures with up to three antennae, sometimes with four N-acetylhexosamine residues in series. There were no major differences between the C. elegans wildtype and hex-5 mutant strains, but the hex-4 mutant strains displayed altered sets of methanol-eluted and PNGase Ar-released pools. In keeping with the specificity of HEX-4, there were more glycans capped with N-acetylgalactosamine in the hex-4 mutants, as compared with isomeric chito-oligomer motifs in the wildtype. Considering that fluorescence microscopy showed that a HEX-4::enhanced GFP fusion protein colocalizes with a Golgi tracker, we conclude that HEX-4 plays a significant role in late-stage Golgi processing of N-glycans in C. elegans. Furthermore, finding more "parasite-like" structures in the model worm may facilitate discovery of glycan-processing enzymes occurring in other nematodes.
|
| 巻・号 |
299(4)
|
| ページ |
103053
|
| 公開日 |
2023-4-1
|
| DOI |
10.1016/j.jbc.2023.103053
|
| PII |
S0021-9258(23)00185-0
|
| PMID |
36813232
|
| PMC |
PMC10060765
|
| MeSH |
Acetylgalactosamine / metabolism
Animals
Caenorhabditis elegans* / metabolism
Glycosylation
Hexosaminidases / metabolism
Methanol
Polysaccharides / metabolism
beta-N-Acetylhexosaminidases* / metabolism
|
| リソース情報 |
| 線虫 |
tm3307 |
