論文 - 詳細
| RRC ID | 76311 |
|---|---|
| 著者 | Tsai HY, Cheng HT, Tsai YT. |
| タイトル | Biogenesis of C. elegans spermatogenesis small RNAs is initiated by a zc3h12a-like ribonuclease. |
| ジャーナル | Sci Adv |
| Abstract |
Small RNAs regulate spermatogenesis across species ranging from Caenorhabditis elegans to humans. In C. elegans, two Argonaute proteins, ALG-3 and ALG-4, and their associated alg-3/4 26G-small RNAs are essential for spermiogenesis at 25°C. The alg-3/4 26G-small RNAs are antisense to their target mRNAs and produced by the RNA-dependent RNA polymerase, RRF-3. However, it remains unclear how the RNA templates for RRF-3 are generated and which cellular processes are affected by alg-3/4 26G-small RNAs. Here, we demonstrate a previously unidentified zc3h12a-like ribonuclease protein, NYN-3, in alg-3/4 26G-small RNAs biogenesis. NYN-3 is not only required for proper abundance of alg-3/4 26G-small RNAs but also crosslinked to their targeted mRNAs before RRF-3 from ePAR-CLIP-seq. Bioinformatics analysis was then used to parse the 26G-small RNA-targeted genes into functional subclasses. Collectively, these findings implicate NYN-3 as an initiator of alg-3/4 26G-small RNA generation. |
| 巻・号 | 8(32) |
| ページ | eabm0699 |
| 公開日 | 2022-8-12 |
| DOI | 10.1126/sciadv.abm0699 |
| PMID | 35947655 |
| PMC | PMC9365287 |
| MeSH | Animals Caenorhabditis elegans* / genetics Caenorhabditis elegans* / metabolism Caenorhabditis elegans Proteins* / genetics Caenorhabditis elegans Proteins* / metabolism Endoribonucleases / metabolism Humans Male RNA, Messenger / genetics RNA, Messenger / metabolism RNA, Small Interfering / metabolism RNA-Binding Proteins / genetics RNA-Binding Proteins / metabolism Ribonucleases / metabolism Spermatogenesis / genetics Transcription Factors / metabolism |
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm2499 tm1156 |