RRC ID 76311
Author Tsai HY, Cheng HT, Tsai YT.
Title Biogenesis of C. elegans spermatogenesis small RNAs is initiated by a zc3h12a-like ribonuclease.
Journal Sci Adv
Abstract Small RNAs regulate spermatogenesis across species ranging from Caenorhabditis elegans to humans. In C. elegans, two Argonaute proteins, ALG-3 and ALG-4, and their associated alg-3/4 26G-small RNAs are essential for spermiogenesis at 25°C. The alg-3/4 26G-small RNAs are antisense to their target mRNAs and produced by the RNA-dependent RNA polymerase, RRF-3. However, it remains unclear how the RNA templates for RRF-3 are generated and which cellular processes are affected by alg-3/4 26G-small RNAs. Here, we demonstrate a previously unidentified zc3h12a-like ribonuclease protein, NYN-3, in alg-3/4 26G-small RNAs biogenesis. NYN-3 is not only required for proper abundance of alg-3/4 26G-small RNAs but also crosslinked to their targeted mRNAs before RRF-3 from ePAR-CLIP-seq. Bioinformatics analysis was then used to parse the 26G-small RNA-targeted genes into functional subclasses. Collectively, these findings implicate NYN-3 as an initiator of alg-3/4 26G-small RNA generation.
Volume 8(32)
Pages eabm0699
Published 2022-8-12
DOI 10.1126/sciadv.abm0699
PMID 35947655
PMC PMC9365287
MeSH Animals Caenorhabditis elegans* / genetics Caenorhabditis elegans* / metabolism Caenorhabditis elegans Proteins* / genetics Caenorhabditis elegans Proteins* / metabolism Endoribonucleases / metabolism Humans Male RNA, Messenger / genetics RNA, Messenger / metabolism RNA, Small Interfering / metabolism RNA-Binding Proteins / genetics RNA-Binding Proteins / metabolism Ribonucleases / metabolism Spermatogenesis / genetics Transcription Factors / metabolism
Resource
C.elegans tm2499 tm1156