| RRC ID |
76327
|
| 著者 |
Wong MK, Ho VWS, Huang X, Chan LY, Xie D, Li R, Ren X, Guan G, Ma Y, Hu B, Yan H, Zhao Z.
|
| タイトル |
Initial characterization of gap phase introduction in every cell cycle of C. elegans embryogenesis.
|
| ジャーナル |
Front Cell Dev Biol
|
| Abstract |
Early embryonic cell cycles usually alternate between S and M phases without any gap phase. When the gap phases are developmentally introduced in various cell types remains poorly defined especially during embryogenesis. To establish the cell-specific introduction of gap phases in embryo, we generate multiple fluorescence ubiquitin cell cycle indicators (FUCCI) in C. elegans. Time-lapse 3D imaging followed by lineal expression profiling reveals sharp and differential accumulation of the FUCCI reporters, allowing the systematic demarcation of cell cycle phases throughout embryogenesis. Accumulation of the reporters reliably identifies both G1 and G2 phases only in two embryonic cells with an extended cell cycle length, suggesting that the remaining cells divide either without a G1 phase, or with a brief G1 phase that is too short to be picked up by our reporters. In summary, we provide an initial picture of gap phase introduction in a metazoan embryo. The newly developed FUCCI reporters pave the way for further characterization of developmental control of cell cycle progression.
|
| 巻・号 |
10
|
| ページ |
978962
|
| 公開日 |
2022-1-1
|
| DOI |
10.3389/fcell.2022.978962
|
| PII |
978962
|
| PMID |
36393848
|
| PMC |
PMC9641140
|
| リソース情報 |
| 線虫 |
tm4063 |
