論文 - 詳細
| RRC ID | 76334 |
|---|---|
| 著者 | Yu X, Gray S, Ferreira HC. |
| タイトル | POT-3 preferentially binds the terminal DNA-repeat on the telomeric G-overhang. |
| ジャーナル | Nucleic Acids Res |
| Abstract |
Eukaryotic chromosomes typically end in 3' telomeric overhangs. The safeguarding of telomeric single-stranded DNA overhangs is carried out by factors related to the protection of telomeres 1 (POT1) protein in humans. Of the three POT1-like proteins in Caenorhabditis elegans, POT-3 was the only member thought to not play a role at telomeres. Here, we provide evidence that POT-3 is a bona fide telomere-binding protein. Using a new loss-of-function mutant, we show that the absence of POT-3 causes telomere lengthening and increased levels of telomeric C-circles. We find that POT-3 directly binds the telomeric G-strand in vitro and map its minimal DNA binding site to the six-nucleotide motif, GCTTAG. We further show that the closely related POT-2 protein binds the same motif, but that POT-3 shows higher sequence selectivity. Crucially, in contrast to POT-2, POT-3 prefers binding sites immediately adjacent to the 3' end of DNA. These differences are significant as genetic analyses reveal that pot-2 and pot-3 do not function redundantly with each other in vivo. Our work highlights the rapid evolution and specialisation of telomere binding proteins and places POT-3 in a unique position to influence activities that control telomere length. |
| 巻・号 | 51(2) |
| ページ | 610-618 |
| 公開日 | 2023-1-25 |
| DOI | 10.1093/nar/gkac1203 |
| PII | 6965455 |
| PMID | 36583365 |
| PMC | PMC9881156 |
| MeSH | Caenorhabditis elegans Proteins* / metabolism DNA / chemistry DNA, Single-Stranded / genetics Humans Shelterin Complex Telomere* / genetics Telomere* / metabolism Telomere-Binding Proteins* / metabolism |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.2 |
| リソース情報 | |
| 線虫 | tm1400 |