論文 - 詳細
| RRC ID | 76336 |
|---|---|
| 著者 | Yuan L, Li P, Jing H, Zheng Q, Xiao H. |
| タイトル | trim-21 promotes proteasomal degradation of CED-1 for apoptotic cell clearance in C. elegans. |
| ジャーナル | Elife |
| Abstract |
The phagocytic receptor CED-1 mediates apoptotic cell recognition by phagocytic cells, enabling cell corpse clearance in Caenorhabditis elegans. Whether appropriate levels of CED-1 are maintained for executing the engulfment function remains unknown. Here, we identified the C. elegans E3 ubiquitin ligase tripartite motif containing-21 (TRIM-21) as a component of the CED-1 pathway for apoptotic cell clearance. When the NPXY motif of CED-1 was bound to the adaptor protein CED-6 or the YXXL motif of CED-1 was phosphorylated by tyrosine kinase SRC-1 and subsequently bound to the adaptor protein NCK-1 containing the SH2 domain, TRIM-21 functioned in conjunction with UBC-21 to catalyze K48-linked poly-ubiquitination on CED-1, targeting it for proteasomal degradation. In the absence of TRIM-21, CED-1 accumulated post-translationally and drove cell corpse degradation defects, as evidenced by direct binding to VHA-10. These findings reveal a unique mechanism for the maintenance of appropriate levels of CED-1 to regulate apoptotic cell clearance. |
| 巻・号 | 11 |
| 公開日 | 2022-8-5 |
| DOI | 10.7554/eLife.76436 |
| PII | 76436 |
| PMID | 35929733 |
| PMC | PMC9388098 |
| MeSH | Animals Apoptosis / physiology Cadaver Caenorhabditis elegans* / physiology Caenorhabditis elegans Proteins* / metabolism Humans Membrane Proteins / metabolism Phagocytosis / physiology |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm847 |