RRC ID 76358
Author Nakajima M, Kawahara R, Simizu S.
Title Cofilin promotes vasculogenic mimicry by regulating the actin cytoskeleton in human breast cancer cells.
Journal FEBS Lett
Abstract Vasculogenic mimicry (VM) is the formation of microvascular channels by cancer cells. VM requires cellular processes that are regulated by changes in cellular migration and morphology. Cofilin (CFL), a key regulator of actin depolymerization, has been reported to affect malignant phenotypes of cancer. We show that treatment with inhibitors of actin dynamics suppresses VM in MDA-MB-231 human breast cancer cells. We established CFL-knockout (KO) MDA-MB-231 cells and found that VM was attenuated in CFL-KO cells. Although the re-expression of wild-type CFL restored VM in CFL-KO cells, inactive phosphomimetic CFL failed to do so. Collectively, our results demonstrate that CFL is a critical regulator of VM and implicate CFL as a novel therapeutic target for breast cancer.
Volume 597(8)
Pages 1114-1124
Published 2023-4-1
DOI 10.1002/1873-3468.14594
PMID 36737242
MeSH Actin Cytoskeleton Actin Depolymerizing Factors Actins Breast Neoplasms* / pathology Cell Line, Tumor Female Humans Neovascularization, Pathologic / genetics
IF 3.057
Human and Animal Cells 293T(RCB2202)
DNA material CSII-CMV-MCS (RDB4377) CSII-CMV-MCS-IRES2-Bsd (RDB4385) pSpCas9n_cofilin sgRNA1 (RDB20017) pSpCas9n_cofilin sgRNA2 (RDB20018) CSII-CMV-MCS_cofilin (RDB20019) CSII-CMV-MCS-IRES2-Bsd_Cas9-resistant cofilin (RDB20020) CSII-CMV-MCS-IRES2-Bsd_Cas10-resistant S3E cofilin (RDB20021)