論文 - 詳細
| RRC ID | 76358 |
|---|---|
| 著者 | Nakajima M, Kawahara R, Simizu S. |
| タイトル | Cofilin promotes vasculogenic mimicry by regulating the actin cytoskeleton in human breast cancer cells. |
| ジャーナル | FEBS Lett |
| Abstract |
Vasculogenic mimicry (VM) is the formation of microvascular channels by cancer cells. VM requires cellular processes that are regulated by changes in cellular migration and morphology. Cofilin (CFL), a key regulator of actin depolymerization, has been reported to affect malignant phenotypes of cancer. We show that treatment with inhibitors of actin dynamics suppresses VM in MDA-MB-231 human breast cancer cells. We established CFL-knockout (KO) MDA-MB-231 cells and found that VM was attenuated in CFL-KO cells. Although the re-expression of wild-type CFL restored VM in CFL-KO cells, inactive phosphomimetic CFL failed to do so. Collectively, our results demonstrate that CFL is a critical regulator of VM and implicate CFL as a novel therapeutic target for breast cancer. |
| 巻・号 | 597(8) |
| ページ | 1114-1124 |
| 公開日 | 2023-4-1 |
| DOI | 10.1002/1873-3468.14594 |
| PMID | 36737242 |
| MeSH | Actin Cytoskeleton Actin Depolymerizing Factors Actins Breast Neoplasms* / pathology Cell Line, Tumor Female Humans Neovascularization, Pathologic / genetics |
| IF | 3.057 |
| リソース情報 | |
| ヒト・動物細胞 | 293T(RCB2202) |
| 遺伝子材料 | CSII-CMV-MCS (RDB4377) CSII-CMV-MCS-IRES2-Bsd (RDB4385) pSpCas9n_cofilin sgRNA1 (RDB20017) pSpCas9n_cofilin sgRNA2 (RDB20018) CSII-CMV-MCS_cofilin (RDB20019) CSII-CMV-MCS-IRES2-Bsd_Cas9-resistant cofilin (RDB20020) CSII-CMV-MCS-IRES2-Bsd_Cas10-resistant S3E cofilin (RDB20021) |