RRC ID 76378
Author Yumura S, Talukder MSU, Pervin MS, Tanvir MIO, Matsumura T, Fujimoto K, Tanaka M, Itoh G.
Title Dynamics of Actin Cytoskeleton and Their Signaling Pathways during Cellular Wound Repair.
Journal Cells
Abstract The repair of wounded cell membranes is essential for cell survival. Upon wounding, actin transiently accumulates at the wound site. The loss of actin accumulation leads to cell death. The mechanism by which actin accumulates at the wound site, the types of actin-related proteins participating in the actin remodeling, and their signaling pathways are unclear. We firstly examined how actin accumulates at a wound site in Dictyostelium cells. Actin assembled de novo at the wound site, independent of cortical flow. Next, we searched for actin- and signal-related proteins targeting the wound site. Fourteen of the examined proteins transiently accumulated at different times. Thirdly, we performed functional analyses using gene knockout mutants or specific inhibitors. Rac, WASP, formin, the Arp2/3 complex, profilin, and coronin contribute to the actin dynamics. Finally, we found that multiple signaling pathways related to TORC2, the Elmo/Doc complex, PIP2-derived products, PLA2, and calmodulin are involved in the actin dynamics for wound repair.
Volume 11(19)
Published 2022-10-9
DOI 10.3390/cells11193166
PII cells11193166
PMID 36231128
PMC PMC9564287
MeSH Actin Cytoskeleton / metabolism Actins* / metabolism Calmodulin / metabolism Dictyostelium* / genetics Dictyostelium* / metabolism Formins Mechanistic Target of Rapamycin Complex 2 / metabolism Phospholipases A2 / metabolism Profilins / genetics Profilins / metabolism Signal Transduction
IF 4.366
Cellular slime molds S00001