Reference - Detail
| RRC ID | 76379 |
|---|---|
| Author | Günther M, Reimer C, Herbst R, Kufs JE, Rautschek J, Ueberschaar N, Zhang S, Peschel G, Reimer L, Regestein L, Valiante V, Hillmann F, Stallforth P. |
| Title | Yellow polyketide pigment suppresses premature hatching in social amoeba. |
| Journal | Proc Natl Acad Sci U S A |
| Abstract |
Low-molecular-weight natural products from microbes are indispensable in the development of potent drugs. However, their biological roles within an ecological context often remain elusive. Here, we shed light on natural products from eukaryotic microorganisms that have the ability to transition from single cells to multicellular organisms: the social amoebae. These eukaryotes harbor a large number of polyketide biosynthetic genes in their genomes, yet virtually none of the corresponding products can be isolated or characterized. Using complementary molecular biology approaches, including CRISPR-Cas9, we generated polyketide synthase (pks5) inactivation and overproduction strains of the social amoeba Dictyostelium discoideum. Differential, untargeted metabolomics of wild-type versus mutant fruiting bodies allowed us to pinpoint candidate metabolites derived from the amoebal PKS5. Extrachromosomal expression of the respective gene led to the identification of a yellow polyunsaturated fatty acid. Analysis of the temporospatial production pattern of this compound in conjunction with detailed bioactivity studies revealed the polyketide to be a spore germination suppressor. |
| Volume | 119(43) |
| Pages | e2116122119 |
| Published | 2022-10-25 |
| DOI | 10.1073/pnas.2116122119 |
| PMID | 36252029 |
| PMC | PMC9618038 |
| MeSH | Amoeba* / genetics Biological Products* / metabolism Dictyostelium* / physiology Polyketide Synthases / genetics Polyketide Synthases / metabolism Polyketides* / metabolism |
| IF | 9.412 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 21 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Cellular slime molds | G90426 |