RRC ID 76380
Author Nguyen LTS, Robinson DN.
Title The lectin Discoidin I acts in the cytoplasm to help assemble the contractile machinery.
Journal J Cell Biol
Abstract Cellular functions, such as division and migration, require cells to undergo robust shape changes. Through their contractility machinery, cells also sense, respond, and adapt to their physical surroundings. In the cytoplasm, the contractility machinery organizes into higher order assemblies termed contractility kits (CKs). Using Dictyostelium discoideum, we previously identified Discoidin I (DscI), a classic secreted lectin, as a CK component through its physical interactions with the actin crosslinker Cortexillin I (CortI) and the scaffolding protein IQGAP2. Here, we find that DscI ensures robust cytokinesis through regulating intracellular components of the contractile machinery. Specifically, DscI is necessary for normal cytokinesis, cortical tension, membrane-cortex connections, and cortical distribution and mechanoresponsiveness of CortI. The dscI deletion mutants also have complex genetic epistatic relationships with CK components, acting as a genetic suppressor of cortI and iqgap1, but as an enhancer of iqgap2. This work underscores the fact that proteins like DiscI contribute in diverse ways to the activities necessary for optimal cell function.
Volume 221(11)
Published 2022-11-7
DOI 10.1083/jcb.202202063
PII 213504
PMID 36165849
PMC PMC9523886
MeSH Actins / metabolism Cytoplasm / metabolism Dictyostelium* / genetics Dictyostelium* / metabolism Discoidins* / metabolism Lectins* / metabolism Microfilament Proteins Protozoan Proteins / genetics Protozoan Proteins / metabolism ras GTPase-Activating Proteins / metabolism
IF 8.811
Cellular slime molds G90426