論文 - 詳細
| RRC ID | 76400 |
|---|---|
| 著者 | Nakamura Y, Matsumoto H, Wu CH, Fukaya D, Uni R, Hirakawa Y, Katagiri M, Yamada S, Ko T, Nomura S, Wada Y, Komuro I, Nangaku M, Inagi R, Inoue T. |
| タイトル | Alpha 7 nicotinic acetylcholine receptors signaling boosts cell-cell interactions in macrophages effecting anti-inflammatory and organ protection. |
| ジャーナル | Commun Biol |
| Abstract |
Activation of the cholinergic anti-inflammatory pathway (CAP) via vagus nerve stimulation has been shown to improve acute kidney injury in rodent models. While alpha 7 nicotinic acetylcholine receptor (α7nAChR) positive macrophages are thought to play a crucial role in this pathway, their in vivo significance has not been fully understood. In this study, we used macrophage-specific α7nAChR-deficient mice to confirm the direct activation of α7nAChRs in macrophages. Our findings indicate that the administration of GTS-21, an α7nAChR-specific agonist, protects injured kidneys in wild-type mice but not in macrophage-specific α7nAChR-deficient mice. To investigate the signal changes or cell reconstructions induced by α7nAChR activation in splenocytes, we conducted single-cell RNA-sequencing of the spleen. Ligand-receptor analysis revealed an increase in macrophage-macrophage interactions. Using macrophage-derived cell lines, we demonstrated that GTS-21 increases cell contact, and that the contact between macrophages receiving α7nAChR signals leads to a reduction in TNF-α. Our results suggest that α7nAChR signaling increases macrophage-macrophage interactions in the spleen and has a protective effect on the kidneys. |
| 巻・号 | 6(1) |
| ページ | 666 |
| 公開日 | 2023-6-23 |
| DOI | 10.1038/s42003-023-05051-2 |
| PII | 10.1038/s42003-023-05051-2 |
| PMID | 37353597 |
| PMC | PMC10290099 |
| MeSH | Animals Anti-Inflammatory Agents / metabolism Cell Communication Macrophages / metabolism Mice Receptors, Nicotinic* / genetics Receptors, Nicotinic* / metabolism alpha7 Nicotinic Acetylcholine Receptor / agonists alpha7 Nicotinic Acetylcholine Receptor / genetics alpha7 Nicotinic Acetylcholine Receptor / metabolism |
| IF | 4.165 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | News |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |