RRC ID 76408
Author Tanaka T, Toujima S, Toyoda S, Takeuchi S, Umesaki N.
Title Establishment and characterization of novel human uterine leiomyosarcoma cell lines.
Journal Int J Oncol
Abstract Patients with unresectable advanced uterine leiomyosarcoma have a very poor prognosis because no effective chemotherapeutic protocols exist. There are currently few established primary human uterine leiomyosarcoma cell lines that can be used to investigate effective therapies. To overcome this problem, we carried out long-term in vitro cell culture and/or nude mouse transplantation and successfully established novel human uterine leiomyosarcoma cell lines from extrauterine and intrauterine tumors that were surgically excised from a single patient. The established cells were characterized by flow cytometry, anticancer drug-sensitivity assays and karyotyping analyses. All the established cell lines showed unstable multiple chromosome abnormalities. Since the cells can proliferate in vitro and in vivo, they will be useful for developing new therapeutic strategies for advanced uterine leiomyosarcoma patients.
Volume 37(1)
Pages 125-31
Published 2010-7-1
DOI 10.3892/ijo_00000660
PMID 20514404
MeSH Adult Algorithms Animals Cell Culture Techniques / methods Cell Line, Tumor Drug Screening Assays, Antitumor Female Flow Cytometry / methods Humans Leiomyosarcoma / pathology* Mice Mice, Inbred BALB C Mice, Nude Neoplasm Transplantation Transplantation, Heterologous Uterine Neoplasms / pathology*
IF 3.899
Resource
Human and Animal Cells SKN(RCB0513)