| RRC ID |
76414
|
| Author |
Mubarok W, Elvitigala KCML, Kotani T, Sakai S.
|
| Title |
Visible light photocrosslinking of sugar beet pectin for 3D bioprinting applications.
|
| Journal |
Carbohydr Polym
|
| Abstract |
Herein, we report the hydrogelation of sugar beet pectin (SBP) via visible light-mediated photocrosslinking and its applications in extrusion-based 3D bioprinting. Rapid hydrogelation (<15 s) was achieved by applying 405 nm visible light to an SBP solution in the presence of tris(bipyridine)ruthenium(II) chloride hexahydrate ([Ru(bpy)3]2+) and sodium persulfate (SPS). The mechanical properties of the hydrogel could be tuned by controlling the visible light irradiation time and concentrations of SBP, [Ru(bpy)3]2+, and SPS. High-fidelity 3D hydrogel constructs were fabricated by extruding inks containing 3.0 wt% SBP, 1.0 mM [Ru(bpy)3]2+, and 1.0 mM SPS. Human hepatoblastoma (HepG2) cells encapsulated in SBP hydrogels remained viable and metabolically active after 14 d of culture. Overall, this study demonstrates the feasibility of applying SBP and a visible light-mediated photocrosslinking system to the 3D bioprinting of cell-laden constructs for tissue engineering applications.
|
| Volume |
316
|
| Pages |
121026
|
| Published |
2023-9-15
|
| DOI |
10.1016/j.carbpol.2023.121026
|
| PII |
S0144-8617(23)00491-5
|
| PMID |
37321724
|
| MeSH |
Beta vulgaris*
Bioprinting*
Humans
Hydrogels
Light
Pectins
Sugars
|
| IF |
7.182
|
| Resource |
| Human and Animal Cells |
Hep G2(RCB1886) |
