| RRC ID |
76417
|
| 著者 |
Takakura Y, Suzuki T, Hirai N, Araki T, Ohishi M, Sato H, Yamaguchi N, Takano H, Yamaguchi N.
|
| タイトル |
VGLL3 confers slow-twitch muscle differentiation via PGC-1α expression in C2C12 myocytes.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
Vestigial-like family member 3 (VGLL3) is a cofactor for the TEA-domain transcription factor (TEAD) family. Although VGLL3 influences myogenic differentiation, its involvement in slow- and fast-twitch fiber specification remains unknown. In this study, we established a cell line stably overexpressing VGLL3 and analyzed effects of VGLL3 on the myogenic differentiation of murine myoblast C2C12 cells. We found that VGLL3 expression promotes slow-twitch muscle differentiation. Mechanistically, VGLL3 expression induced the expression of peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α), a master transcriptional regulator of slow-twitch muscle development. We also found that VGLL3 proteins are degraded by the proteasome, which causes switching of TEAD cofactors from VGLL3 to Yes-associated protein (YAP) and transcriptional coactivator with a PDZ-binding motif (TAZ). These results suggest that the balance between the two kinds of TEAD cofactors VGLL3 and YAP/TAZ controls muscle fiber-type specification.
|
| 巻・号 |
669
|
| ページ |
30-37
|
| 公開日 |
2023-8-20
|
| DOI |
10.1016/j.bbrc.2023.05.073
|
| PII |
S0006-291X(23)00651-4
|
| PMID |
37262950
|
| MeSH |
Animals
Cell Differentiation
Gene Expression Regulation
Mice
Muscle Fibers, Skeletal* / metabolism
Muscle Fibers, Slow-Twitch / metabolism
Muscle, Skeletal / metabolism
Myoblasts / metabolism
Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha* / genetics
Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha* / metabolism
Transcription Factors* / metabolism
|
| IF |
2.985
|
| リソース情報 |
| ヒト・動物細胞 |
C2C12(RCB0987) |
