論文 - 詳細
| RRC ID | 76417 |
|---|---|
| 著者 | Takakura Y, Suzuki T, Hirai N, Araki T, Ohishi M, Sato H, Yamaguchi N, Takano H, Yamaguchi N. |
| タイトル | VGLL3 confers slow-twitch muscle differentiation via PGC-1α expression in C2C12 myocytes. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Vestigial-like family member 3 (VGLL3) is a cofactor for the TEA-domain transcription factor (TEAD) family. Although VGLL3 influences myogenic differentiation, its involvement in slow- and fast-twitch fiber specification remains unknown. In this study, we established a cell line stably overexpressing VGLL3 and analyzed effects of VGLL3 on the myogenic differentiation of murine myoblast C2C12 cells. We found that VGLL3 expression promotes slow-twitch muscle differentiation. Mechanistically, VGLL3 expression induced the expression of peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α), a master transcriptional regulator of slow-twitch muscle development. We also found that VGLL3 proteins are degraded by the proteasome, which causes switching of TEAD cofactors from VGLL3 to Yes-associated protein (YAP) and transcriptional coactivator with a PDZ-binding motif (TAZ). These results suggest that the balance between the two kinds of TEAD cofactors VGLL3 and YAP/TAZ controls muscle fiber-type specification. |
| 巻・号 | 669 |
| ページ | 30-37 |
| 公開日 | 2023-8-20 |
| DOI | 10.1016/j.bbrc.2023.05.073 |
| PII | S0006-291X(23)00651-4 |
| PMID | 37262950 |
| MeSH | Animals Cell Differentiation Gene Expression Regulation Mice Muscle Fibers, Skeletal* / metabolism Muscle Fibers, Slow-Twitch / metabolism Muscle, Skeletal / metabolism Myoblasts / metabolism Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha* / genetics Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha* / metabolism Transcription Factors* / metabolism |
| IF | 2.985 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | C2C12(RCB0987) |