RRC ID 76449
Author Tounosu N, Sesoko K, Hori K, Shimojima M, Ohta H.
Title Cis-regulatory elements and transcription factors related to auxin signaling in the streptophyte algae Klebsormidium nitens.
Journal Sci Rep
Abstract The phytohormone auxin affects numerous processes in land plants. The central auxin signaling machinery, called the nuclear auxin pathway, is mediated by its pivotal receptor named TRANSPORT INHIBITOR RESPONSE 1/AUXIN SIGNALING F-BOX (TIR1/AFB). The nuclear auxin pathway is widely conserved in land plants, but auxin also accumulates in various algae. Although auxin affects the growth of several algae, the components that mediate auxin signaling have not been identified. We previously reported that exogenous auxin suppresses cell proliferation in the Klebsormidium nitens that is a member of streptophyte algae, a paraphyletic group sharing the common ancestor with land plants. Although K. nitens lacks TIR1/AFB, auxin affects the expression of numerous genes. Thus, elucidation of the mechanism of auxin-inducible gene expression in K. nitens would provide important insights into the evolution of auxin signaling. Here, we show that some motifs are enriched in the promoter sequences of auxin-inducible genes in K. nitens. We also found that the transcription factor KnRAV activates several auxin-inducible genes and directly binds the promoter of KnLBD1, a representative auxin-inducible gene. We propose that KnRAV has the potential to regulate auxin-responsive gene expression in K. nitens.
Volume 13(1)
Pages 9635
Published 2023-6-15
DOI 10.1038/s41598-023-36500-x
PII 10.1038/s41598-023-36500-x
PMID 37322074
PMC PMC10272232
MeSH Arabidopsis Proteins* / genetics F-Box Proteins* / genetics Gene Expression Regulation, Plant Indoleacetic Acids / metabolism Plant Growth Regulators Plants / metabolism Streptophyta* / metabolism Transcription Factors / genetics Transcription Factors / metabolism
IF 3.998
Algae NIES-2285