RRC ID 76493
Author Guo C, Webb SE, Chan CM, Miller AL.
Title TPC2-mediated Ca2+ signaling is required for axon extension in caudal primary motor neurons in zebrafish embryos.
Journal J Cell Sci
Abstract The role of two-pore channel type 2 (TPC2, encoded by tcpn2)-mediated Ca2+ release was recently characterized in zebrafish during establishment of the early spinal circuitry, one of the key events in the coordination of neuromuscular activity. Here, we extend our study to investigate the in vivo role of TPC2 in the regulation of caudal primary motor neuron (CaP) axon extension. We used a combination of TPC2 knockdown with a translation-blocking morpholino antisense oligonucleotide (MO), TPC2 knockout via the generation of a tpcn2dhkz1a mutant line of zebrafish using CRISPR/Cas9 gene-editing and pharmacological inhibition of TPC2 via incubation with bafilomycin A1 (an H+-ATPase inhibitor) or trans-ned-19 (an NAADP receptor antagonist), and showed that these treatments attenuated CaP Ca2+ signaling and inhibited axon extension. We also characterized the expression of an arc1-like transcript in CaPs grown in primary culture. MO-mediated knockdown of ARC1-like in vivo led to attenuation of the Ca2+ transients in the CaP growth cones and an inhibition of axon extension. Together, our new data suggest a link between ARC1-like, TPC2 and Ca2+ signaling during axon extension in zebrafish.
Volume 133(13)
Published 2020-7-10
DOI 10.1242/jcs.244780
PII jcs.244780
PMID 32546534
MeSH Animals Axons / metabolism Calcium / metabolism Calcium Channels* Motor Neurons / metabolism Zebrafish* / genetics Zebrafish* / metabolism
IF 4.573
Zebrafish SAIGFF213A, UAS:GCaMP7a, UAS:GFP