Reference - Detail
| RRC ID | 76527 |
|---|---|
| Author | Nguyen PTM, Ishiwata-Kimata Y, Kimata Y. |
| Title | Monitoring ADP/ATP ratio in yeast cells using the fluorescent-protein reporter PercevalHR. |
| Journal | Biosci Biotechnol Biochem |
| Abstract |
PercevalHR (Perceval High Resolution) is an artificially designed fluorescent protein, which changes its excitation spectrum based on the ADP/ATP ratio of the environment. Here we demonstrated that PercevalHR can be used for monitoring energy status of Saccharomyces cerevisiae cells, which are affected by diauxic shift and mitochondria inhibition, in a non-invasive and non-destructive manner. |
| Volume | 83(5) |
| Pages | 824-828 |
| Published | 2019-5-1 |
| DOI | 10.1080/09168451.2019.1574204 |
| PMID | 30704350 |
| MeSH | Adenosine Diphosphate / metabolism* Adenosine Triphosphate / metabolism* Fluorescence Luminescent Proteins / metabolism* Mitochondria / metabolism Saccharomyces cerevisiae / metabolism* |
| IF | 1.516 |
| Resource | |
| Yeast | BYP9646 |