論文 - 詳細
| RRC ID | 76602 |
|---|---|
| 著者 | Maki T, Thon G, Iwasaki H. |
| タイトル | Fission yeast Swi2 designates cell-type specific donor and stimulates Rad51-driven strand exchange for mating-type switching. |
| ジャーナル | Nucleic Acids Res |
| Abstract |
A haploid of the fission yeast Schizosaccharomyces pombe expresses either the P or M mating-type, determined by the active, euchromatic, mat1 cassette. Mating-type is switched by Rad51-driven gene conversion of mat1 using a heterochromatic donor cassette, mat2-P or mat3-M. The Swi2-Swi5 complex, a mating-type switching factor, is central to this process by designating a preferred donor in a cell-type-specific manner. Swi2-Swi5 selectively enables one of two cis-acting recombination enhancers, SRE2 adjacent to mat2-P or SRE3 adjacent to mat3-M. Here, we identified two functionally important motifs in Swi2, a Swi6 (HP1 homolog)-binding site and two DNA-binding AT-hooks. Genetic analysis demonstrated that the AT-hooks were required for Swi2 localization at SRE3 to select the mat3-M donor in P cells, while the Swi6-binding site was required for Swi2 localization at SRE2 to select mat2-P in M cells. In addition, the Swi2-Swi5 complex promoted Rad51-driven strand exchange in vitro. Taken together, our results show how the Swi2-Swi5 complex would localize to recombination enhancers through a cell-type specific binding mechanism and stimulate Rad51-driven gene conversion at the localization site. |
| 巻・号 | 51(8) |
| ページ | 3869-3887 |
| 公開日 | 2023-5-8 |
| DOI | 10.1093/nar/gkad204 |
| PII | 7084606 |
| PMID | 36951094 |
| PMC | PMC10164574 |
| MeSH | DNA, Fungal / genetics DNA, Fungal / metabolism Gene Conversion Genes, Fungal Genes, Mating Type, Fungal / genetics Recombination, Genetic Schizosaccharomyces* / genetics Schizosaccharomyces* / metabolism Schizosaccharomyces pombe Proteins* / genetics Schizosaccharomyces pombe Proteins* / metabolism |
| IF | 11.502 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 酵母 | FY47735 FY47736 FY47737 FY47738 FY47739 FY47740 FY47741 FY44265 |