| RRC ID |
76620
|
| Author |
Minami S, Matsumoto N, Omori H, Nakamura Y, Tamiya S, Nouda R, Nurdin JA, Yamasaki M, Kotaki T, Kanai Y, Okamoto T, Tachibana T, Ushijima H, Kobayashi T, Sato S.
|
| Title |
Effective SARS-CoV-2 replication of monolayers of intestinal epithelial cells differentiated from human induced pluripotent stem cells.
|
| Journal |
Sci Rep
|
| Abstract |
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes severe acute respiratory symptoms in humans. Controlling the coronavirus disease pandemic is a worldwide priority. The number of SARS-CoV-2 studies has dramatically increased, and the requirement for analytical tools is higher than ever. Here, we propose monolayered-intestinal epithelial cells (IECs) derived from human induced pluripotent stem cells (iPSCs) instead of three-dimensional cultured intestinal organoids as a suitable tool to study SARS-CoV-2 infection. Differentiated IEC monolayers express high levels of angiotensin-converting enzyme 2 and transmembrane protease serine 2 (TMPRSS2), host factors essential for SARS-CoV-2 infection. SARS-CoV-2 efficiently grows in IEC monolayers. Using this propagation system, we confirm that TMPRSS2 inhibition blocked SARS-CoV-2 infection in IECs. Hence, our iPSC-derived IEC monolayers are suitable for SARS-CoV-2 research under physiologically relevant conditions.
|
| Volume |
13(1)
|
| Pages |
11610
|
| Published |
2023-7-18
|
| DOI |
10.1038/s41598-023-38548-1
|
| PII |
10.1038/s41598-023-38548-1
|
| PMID |
37463955
|
| PMC |
PMC10354114
|
| MeSH |
COVID-19*
Epithelial Cells
Humans
Induced Pluripotent Stem Cells*
Intestines
SARS-CoV-2
|
| IF |
3.998
|
| Resource |
| Human and Animal Cells |
1231A3(HPS0381)
1383D6(HPS1006) |
