RRC ID 76632
Author Saito R, Otsu M, Kiyosawa H, Kawai G.
Title Expression analysis of box C/D snoRNAs with SNPs between C57BL/6 and MSM/Ms strains in male mouse.
Journal PLoS One
Abstract MSM/Ms mouse derived from the Japanese wild mouse has unique characteristics compared to the widely used C57BL/6 mouse. To examine the usefulness of the MSM/Ms mouse for the comparative genomic analysis, expression of small RNAs were analyzed by the large-scale sequence analysis for two strains of mouse, C57BL/6 and MSM/Ms. As a trial, expression of box C/D snoRNAs, which are the most abundant small RNAs in the cell, were analyzed. By the comparison of the read number for each fragment, 11 snoRNAs with single nucleotide polymorphisms (SNPs) were detected. One of the snoRNAs, SNORD53, shows the expression only for MSM/Ms and this snoRNA has a mutation in the box sequence in C57BL/6. Thus, it was demonstrated that the proposed experimental system using SNPs can give new insight for the gene expression regulation.
Volume 18(7)
Pages e0288362
Published 2023-1-1
DOI 10.1371/journal.pone.0288362
PII PONE-D-23-05631
PMID 37428787
PMC PMC10332580
MeSH Animals Base Sequence Homosexuality, Male Humans Male Mice Mice, Inbred C57BL Polymorphism, Single Nucleotide RNA, Small Nucleolar* / genetics Sexual and Gender Minorities*
IF 2.74
Mice RBRC00743