RRC ID 76652
Author Nomura A, Kobayashi T, Seo W, Ohno-Oishi M, Kakugawa K, Muroi S, Yoshida H, Endo TA, Moro K, Taniuchi I.
Title Identification of a novel enhancer essential for Satb1 expression in TH2 cells and activated ILC2s.
Journal Life Sci Alliance
Abstract The genome organizer, special AT-rich binding protein-1 (SATB1), functions to globally regulate gene networks during primary T cell development and plays a pivotal role in lineage specification in CD4+ helper-, CD8+ cytotoxic-, and FOXP3+ regulatory-T cell subsets. However, it remains unclear how Satb1 gene expression is controlled, particularly in effector T cell function. Here, by using a novel reporter mouse strain expressing SATB1-Venus and genome editing, we have identified a cis-regulatory enhancer, essential for maintaining Satb1 expression specifically in TH2 cells. This enhancer is occupied by STAT6 and interacts with Satb1 promoters through chromatin looping in TH2 cells. Reduction of Satb1 expression, by the lack of this enhancer, resulted in elevated IL-5 expression in TH2 cells. In addition, we found that Satb1 is induced in activated group 2 innate lymphoid cells (ILC2s) through this enhancer. Collectively, these results provide novel insights into how Satb1 expression is regulated in TH2 cells and ILC2s during type 2 immune responses.
Volume 6(8)
Published 2023-8-1
DOI 10.26508/lsa.202301897
PII 6/8/e202301897
PMID 37193606
PMC PMC10189277
MeSH Animals Cell Differentiation Immunity, Innate Lymphocytes Matrix Attachment Region Binding Proteins* / genetics Matrix Attachment Region Binding Proteins* / metabolism Mice Transcription Factors / genetics Transcription Factors / metabolism
IF 2.622
DNA material B6N Mouse BAC clone (RDB07573) B6Ng01-312L13