| RRC ID |
76702
|
| Author |
Sakai E, Imaizumi T, Suzuki R, Taracena-Gándara M, Fujimoto T, Sakurai F, Mizuguchi H.
|
| Title |
miR-27b targets MAIP1 to mediate lipid accumulation in cultured human and mouse hepatic cells.
|
| Journal |
Commun Biol
|
| Abstract |
Non-alcoholic liver disease (NAFLD) is a condition caused by excessive fat accumulation in the liver and developed via multiple pathways. miR-27b has been suggested to play crucial roles in the development of NAFLD, assuming via targeting genes involved in lipid catabolism and anabolism. However, other pathways regulated by miR-27b are largely unknown. Here we show that lipid accumulation was induced in miR-27b-transfected human and mouse hepatic cells and that knockdowns of three miR-27b-target genes, β-1,4-galactosyltransferase 3 (B4GALT3), matrix AAA peptidase interacting protein 1 (MAIP1) and PH domain and leucine rich repeat protein phosphatase 2 (PHLPP2), induced lipid accumulation. We also show that B4GALT3 and MAIP1 were direct targets of miR-27b and overexpression of MAIP1 ameliorated miR-27b-induced lipid accumulation. In addition, we show that hepatic Maip1 expression declined in mice fed a high-fat diet, suggesting the involvement of decreased Maip1 expression in the condition of fatty liver. Overall, we identified MAIP1/miR-27b axis as a mediator of hepatic lipid accumulation, a potential therapeutic target for NAFLD.
|
| Volume |
6(1)
|
| Pages |
669
|
| Published |
2023-6-24
|
| DOI |
10.1038/s42003-023-05049-w
|
| PII |
10.1038/s42003-023-05049-w
|
| PMID |
37355744
|
| PMC |
PMC10290684
|
| MeSH |
Animals
Hepatocytes / metabolism
Humans
Lipid Metabolism / genetics
Lipids
Mice
MicroRNAs* / genetics
MicroRNAs* / metabolism
Non-alcoholic Fatty Liver Disease* / genetics
Non-alcoholic Fatty Liver Disease* / metabolism
Phosphoprotein Phosphatases / metabolism
|
| IF |
4.165
|
| Resource |
| DNA material |
CSII-EF-IRES2-Venus (RDB04384)
CSII-EF-MCS (RDB04378)
pCMV-VSVG-RSV-Rev (RDB04393)
pCAG-HIVgp (RDB04394) |
