論文 - 詳細
| RRC ID | 76702 |
|---|---|
| 著者 | Sakai E, Imaizumi T, Suzuki R, Taracena-Gándara M, Fujimoto T, Sakurai F, Mizuguchi H. |
| タイトル | miR-27b targets MAIP1 to mediate lipid accumulation in cultured human and mouse hepatic cells. |
| ジャーナル | Commun Biol |
| Abstract |
Non-alcoholic liver disease (NAFLD) is a condition caused by excessive fat accumulation in the liver and developed via multiple pathways. miR-27b has been suggested to play crucial roles in the development of NAFLD, assuming via targeting genes involved in lipid catabolism and anabolism. However, other pathways regulated by miR-27b are largely unknown. Here we show that lipid accumulation was induced in miR-27b-transfected human and mouse hepatic cells and that knockdowns of three miR-27b-target genes, β-1,4-galactosyltransferase 3 (B4GALT3), matrix AAA peptidase interacting protein 1 (MAIP1) and PH domain and leucine rich repeat protein phosphatase 2 (PHLPP2), induced lipid accumulation. We also show that B4GALT3 and MAIP1 were direct targets of miR-27b and overexpression of MAIP1 ameliorated miR-27b-induced lipid accumulation. In addition, we show that hepatic Maip1 expression declined in mice fed a high-fat diet, suggesting the involvement of decreased Maip1 expression in the condition of fatty liver. Overall, we identified MAIP1/miR-27b axis as a mediator of hepatic lipid accumulation, a potential therapeutic target for NAFLD. |
| 巻・号 | 6(1) |
| ページ | 669 |
| 公開日 | 2023-6-24 |
| DOI | 10.1038/s42003-023-05049-w |
| PII | 10.1038/s42003-023-05049-w |
| PMID | 37355744 |
| PMC | PMC10290684 |
| MeSH | Animals Hepatocytes / metabolism Humans Lipid Metabolism / genetics Lipids Mice MicroRNAs* / genetics MicroRNAs* / metabolism Non-alcoholic Fatty Liver Disease* / genetics Non-alcoholic Fatty Liver Disease* / metabolism Phosphoprotein Phosphatases / metabolism |
| IF | 4.165 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | CSII-EF-IRES2-Venus (RDB04384) CSII-EF-MCS (RDB04378) pCMV-VSVG-RSV-Rev (RDB04393) pCAG-HIVgp (RDB04394) |