論文 - 詳細
| RRC ID | 76820 |
|---|---|
| 著者 | Yamada T, Yamamori Y, Matsuda N, Nagamune H, Ohkura K, Tomoyasu T, Tabata A. |
| タイトル | Streptolysin S induces pronounced calcium-ion influx-dependent expression of immediate early genes encoding transcription factors. |
| ジャーナル | Sci Rep |
| Abstract |
Anginosus group streptococci (AGS) are opportunistic human pathogens of the oral cavity. The β-hemolytic subgroup of Streptococcus anginosus subsp. anginosus secretes streptolysin S (SLS) and exhibits not only hemolytic activity but also cytotoxicity toward cultured human cell lines. However, the detailed mechanism of action of SLS and the cellular responses of host cells have not yet been fully clarified. To determine the pathogenic potential of SLS-producing β-hemolytic S. anginosus subsp. anginosus, the SLS-dependent response induced in the human oral squamous cell carcinoma HSC-2 cells was investigated to determine the pathogenic potential of SLS-producing β-hemolytic S. anginosus subsp. anginosus. This study revealed that the Ca2+ influx and the expression of immediate early genes (IEGs) encoding transcription factors such as early growth responses (EGRs) and activator protein-1 (AP-1) were greatly increased in HSC-2 cells incubated with the culture supernatant of SLS-producing β-hemolytic S. anginosus subsp. anginosus. Moreover, this SLS-dependent increase in expression was significantly suppressed by Ca2+ chelation, except for jun. These results suggest that SLS caused Ca2+ influx into the cells following greatly enhanced expression of IEG-encoding transcription factors. The results of this study may help in understanding the pathogenicity of SLS-producing AGS. |
| 巻・号 | 13(1) |
| ページ | 13720 |
| 公開日 | 2023-8-22 |
| DOI | 10.1038/s41598-023-40981-1 |
| PII | 10.1038/s41598-023-40981-1 |
| PMID | 37608082 |
| PMC | PMC10444759 |
| MeSH | Betaproteobacteria* Calcium Carcinoma, Squamous Cell* Genes, Immediate-Early Head and Neck Neoplasms* Humans Ions Mouth Neoplasms* Streptococcus pyogenes Transcription Factor AP-1 |
| IF | 3.998 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HSC-2(RCB1945) |