| RRC ID |
76827
|
| 著者 |
Shiraki N, Maruyama K, Hayashi R, Oguchi A, Murakawa Y, Katayama T, Takigawa T, Sakimoto S, Quantock AJ, Tsujikawa M, Nishida K.
|
| タイトル |
PAX6-positive microglia evolve locally in hiPSC-derived ocular organoids.
|
| ジャーナル |
Stem Cell Reports
|
| Abstract |
Microglia are the resident immune cells of the central nervous system (CNS). They govern the immunogenicity of the retina, which is considered to be part of the CNS; however, it is not known how microglia develop in the eye. Here, we studied human-induced pluripotent stem cells (hiPSCs) that had been expanded into a self-formed ectodermal autonomous multi-zone (SEAM) of cells that partially mimics human eye development. Our results indicated that microglia-like cells, which have characteristics of yolk-sac-like linage cells, naturally develop in 2D eye-like SEAM organoids, which lack any vascular components. These cells are unique in that they are paired box protein 6 (PAX6)-positive, yet they possess some characteristics of mesoderm. Collectively, the data support the notion of the existence of an isolated, locally developing immune system in the eye, which is independent of the body's vasculature and general immune system.
|
| 巻・号 |
17(2)
|
| ページ |
221-230
|
| 公開日 |
2022-2-8
|
| DOI |
10.1016/j.stemcr.2021.12.009
|
| PII |
S2213-6711(21)00648-2
|
| PMID |
35030319
|
| PMC |
PMC8828554
|
| MeSH |
Cytokines / pharmacology
Eye / cytology
Eye / growth & development
Gene Expression Regulation / drug effects
Humans
Induced Pluripotent Stem Cells / cytology
Induced Pluripotent Stem Cells / metabolism
Lipopolysaccharides / pharmacology
Microglia / metabolism*
Organoids / cytology
Organoids / metabolism
PAX6 Transcription Factor / genetics
PAX6 Transcription Factor / metabolism*
Phagocytosis
|
| IF |
6.032
|
| リソース情報 |
| ヒト・動物細胞 |
201B7(HPS0063)
KhES-1(HES0001) |
