論文 - 詳細
| RRC ID | 76827 |
|---|---|
| 著者 | Shiraki N, Maruyama K, Hayashi R, Oguchi A, Murakawa Y, Katayama T, Takigawa T, Sakimoto S, Quantock AJ, Tsujikawa M, Nishida K. |
| タイトル | PAX6-positive microglia evolve locally in hiPSC-derived ocular organoids. |
| ジャーナル | Stem Cell Reports |
| Abstract |
Microglia are the resident immune cells of the central nervous system (CNS). They govern the immunogenicity of the retina, which is considered to be part of the CNS; however, it is not known how microglia develop in the eye. Here, we studied human-induced pluripotent stem cells (hiPSCs) that had been expanded into a self-formed ectodermal autonomous multi-zone (SEAM) of cells that partially mimics human eye development. Our results indicated that microglia-like cells, which have characteristics of yolk-sac-like linage cells, naturally develop in 2D eye-like SEAM organoids, which lack any vascular components. These cells are unique in that they are paired box protein 6 (PAX6)-positive, yet they possess some characteristics of mesoderm. Collectively, the data support the notion of the existence of an isolated, locally developing immune system in the eye, which is independent of the body's vasculature and general immune system. |
| 巻・号 | 17(2) |
| ページ | 221-230 |
| 公開日 | 2022-2-8 |
| DOI | 10.1016/j.stemcr.2021.12.009 |
| PII | S2213-6711(21)00648-2 |
| PMID | 35030319 |
| PMC | PMC8828554 |
| MeSH | Cytokines / pharmacology Eye / cytology Eye / growth & development Gene Expression Regulation / drug effects Humans Induced Pluripotent Stem Cells / cytology Induced Pluripotent Stem Cells / metabolism Lipopolysaccharides / pharmacology Microglia / metabolism* Organoids / cytology Organoids / metabolism PAX6 Transcription Factor / genetics PAX6 Transcription Factor / metabolism* Phagocytosis |
| IF | 6.032 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 16 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 201B7(HPS0063) KhES-1(HES0001) |