RRC ID 76827
Author Shiraki N, Maruyama K, Hayashi R, Oguchi A, Murakawa Y, Katayama T, Takigawa T, Sakimoto S, Quantock AJ, Tsujikawa M, Nishida K.
Title PAX6-positive microglia evolve locally in hiPSC-derived ocular organoids.
Journal Stem Cell Reports
Abstract Microglia are the resident immune cells of the central nervous system (CNS). They govern the immunogenicity of the retina, which is considered to be part of the CNS; however, it is not known how microglia develop in the eye. Here, we studied human-induced pluripotent stem cells (hiPSCs) that had been expanded into a self-formed ectodermal autonomous multi-zone (SEAM) of cells that partially mimics human eye development. Our results indicated that microglia-like cells, which have characteristics of yolk-sac-like linage cells, naturally develop in 2D eye-like SEAM organoids, which lack any vascular components. These cells are unique in that they are paired box protein 6 (PAX6)-positive, yet they possess some characteristics of mesoderm. Collectively, the data support the notion of the existence of an isolated, locally developing immune system in the eye, which is independent of the body's vasculature and general immune system.
Volume 17(2)
Pages 221-230
Published 2022-2-8
DOI 10.1016/j.stemcr.2021.12.009
PII S2213-6711(21)00648-2
PMID 35030319
PMC PMC8828554
MeSH Cytokines / pharmacology Eye / cytology Eye / growth & development Gene Expression Regulation / drug effects Humans Induced Pluripotent Stem Cells / cytology Induced Pluripotent Stem Cells / metabolism Lipopolysaccharides / pharmacology Microglia / metabolism* Organoids / cytology Organoids / metabolism PAX6 Transcription Factor / genetics PAX6 Transcription Factor / metabolism* Phagocytosis
IF 6.032
Resource
Human and Animal Cells 201B7(HPS0063) KhES-1(HES0001)