論文 - 詳細
| RRC ID | 76830 |
|---|---|
| 著者 | Tagawa Y, Sakagami H, Tanuma SI, Amano S, Uota S, Bandow K, Tomomura M, Uesawa Y, Takao K, Sugita Y, Yamamoto N, Sakashita H, Nakakaji R, Koizumi T, Mitsudo K, Tohnai I. |
| タイトル | Potentiation of Anticancer Activity of G2/M Blockers by Mild Hyperthermia. |
| ジャーナル | Anticancer Res |
| Abstract |
BACKGROUND/AIM:Hyperthermia (HT), combined with chemotherapy, has been used to treat various types of cancer. This study aimed to investigate the HT-sensitivity of malignant and non-malignant cells, and then evaluate the combination effect of docetaxel (DTX) and a newly synthesized chromone derivative (compound A) with HT. MATERIALS AND METHODS:The number of viable cells was determined using the MTT method. Cell cycle distribution was analyzed using a cell sorter, and DNA fragmentation pattern was detected using agarose gel electrophoresis. RESULTS:Among 12 cultured cells, oral squamous cell carcinoma (OSCC), especially Ca9-22 cells, and myelogenous leukemia cells showed higher sensitivity to HT than lung carcinoma and glioblastoma cell lines, while normal oral cells were the most resistant. Cytotoxicity of DTX on Ca9-22 cells was maximum at 41-42°C and 45~60 min exposure to HT. DXT, compound A, and HT induced G2/M arrest of Ca-22 cells. Mild HT enhanced the DTX- and compound A-induced subG1 arrest, in a synergistic fashion. CONCLUSION:The combination G2/M blockers and mild-HT can potentially be used for the treatment of OSCC. |
| 巻・号 | 43(8) |
| ページ | 3429-3439 |
| 公開日 | 2023-8-1 |
| DOI | 10.21873/anticanres.16518 |
| PII | 43/8/3429 |
| PMID | 37500171 |
| MeSH | Apoptosis Carcinoma, Squamous Cell* / drug therapy Cell Line, Tumor Docetaxel / pharmacology Docetaxel / therapeutic use Humans Hyperthermia, Induced* Mouth Neoplasms* / drug therapy |
| IF | 1.994 |
| リソース情報 | |
| ヒト・動物細胞 | Ca9-22(RCB1976) HSC-2(RCB1945) HSC-3(RCB1975) HSC-4(RCB1902) A904L(RCB3531) T98G(RCB1954) A549(RCB0098) WA-hT(RCB2279) |