論文 - 詳細
| RRC ID | 76838 |
|---|---|
| 著者 | Tamaki S, Koshitsuka Y, Miyamoto K, Ishikawa T, Shinomura T. |
| タイトル | Chemical and genetic carotenoid deficiency delays growth in dark-grown Euglena gracilis. |
| ジャーナル | Biosci Biotechnol Biochem |
| Abstract |
Light-independent functions of carotenoids in photosynthetic organisms are poorly understood. Here, we investigated the growth properties of microalga, Euglena gracilis, under altered light and temperature using norflurazon-treated carotenoid-deficient cells and genetically modified strains, including nonphotosynthetic SM-ZK and colorless cl4. Norflurazon treatment decreased carotenoid and chlorophyll contents, causing cell bleaching. SM-ZK strain had lower carotenoid content than wild-type (WT) strain, and it was below the detectable level in the cl4 strain. Norflurazon treatment decreased phytoene synthase EgCrtB levels, although EgcrtB was transcriptionally induced. Carotenoid deficiency in norflurazon-treated cells and the cl4 strain caused similar extents of delayed growth under light and dark conditions at 25 °C, indicating that carotenoids promote growth in darkness. Both WT and SM-ZK strains exhibited similar growth rates. Dark conditions at 20 °C enhanced the growth delay of norflurazon-treated cells and the cl4 strain. These results indicate that carotenoids impart environmental stress tolerance to E. gracilis in light-dependent and light-independent manners. |
| 巻・号 | 87(5) |
| ページ | 491-500 |
| 公開日 | 2023-4-24 |
| DOI | 10.1093/bbb/zbad024 |
| PII | 7069318 |
| PMID | 36869792 |
| MeSH | Carotenoids Chlorophyll Darkness Euglena* Euglena gracilis* Light |
| IF | 1.516 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 藻類 | NIES-48 |