| RRC ID |
76851
|
| 著者 |
Yamada T, Yoshinari Y, Tobo M, Habara O, Nishimura T.
|
| タイトル |
Nacα protects the larval fat body from cell death by maintaining cellular proteostasis in Drosophila.
|
| ジャーナル |
Nat Commun
|
| Abstract |
Protein homeostasis (proteostasis) is crucial for the maintenance of cellular homeostasis. Impairment of proteostasis activates proteotoxic and unfolded protein response pathways to resolve cellular stress or induce apoptosis in damaged cells. However, the responses of individual tissues to proteotoxic stress and evoking cell death program have not been extensively explored in vivo. Here, we show that a reduction in Nascent polypeptide-associated complex protein alpha subunit (Nacα) specifically and progressively induces cell death in Drosophila fat body cells. Nacα mutants disrupt both ER integrity and the proteasomal degradation system, resulting in caspase activation through JNK and p53. Although forced activation of the JNK and p53 pathways was insufficient to induce cell death in the fat body, the reduction of Nacα sensitized fat body cells to intrinsic and environmental stresses. Reducing overall protein synthesis by mTor inhibition or Minute mutants alleviated the cell death phenotype in Nacα mutant fat body cells. Our work revealed that Nacα is crucial for protecting the fat body from cell death by maintaining cellular proteostasis, thus demonstrating the coexistence of a unique vulnerability and cell death resistance in the fat body.
|
| 巻・号 |
14(1)
|
| ページ |
5328
|
| 公開日 |
2023-9-1
|
| DOI |
10.1038/s41467-023-41103-1
|
| PII |
10.1038/s41467-023-41103-1
|
| PMID |
37658058
|
| PMC |
PMC10474126
|
| MeSH |
Animals
Cell Death
Drosophila*
Fat Body
Larva
Proteostasis*
Tumor Suppressor Protein p53
|
| IF |
12.121
|
| リソース情報 |
| ショウジョウバエ |
8759R-1
3644R-1
HMS00752
DGRC#102285
DGRC#108011
DGRC#111396
DGRC#114620 |
