論文 - 詳細
| RRC ID | 76855 |
|---|---|
| 著者 | Yamada M, Sofuni T, Nohmi T. |
| タイトル | Preferential induction of AT-TA transversion, but not deletions, by chlorambucil at the hisG428 site of Salmonella typhimurium TA102. |
| ジャーナル | Mutat Res |
| Abstract |
The chemotherapeutic agent chlorambucil effectively induces deletion mutations in mouse germ cells. The possibility that this chemical also effectively induces deletion mutations in bacterial DNA was examined using Ames Salmonella tester strains. Chlorambucil was mutagenic only to strains TA102 (hisG428, rfa/pKM101) and YG2975 (hisG46, rfa/pKM101) when S9 mix was absent. Since strain TA102 can detect short deletions, the mutational changes of TA102 induced by this agent without S9 mix were directly determined by the DNA sequencing technique. It turned out that chlorambucil did not induce deletion mutations but preferentially induced AT-TA transversions at the hisG428 site of plasmid pAQ1 of strain TA102. These results caution that the positive results induced by chlorambucil in mutagenicity tests do not necessarily mean the occurrence of deletions. |
| 巻・号 | 283(1) |
| ページ | 29-33 |
| 公開日 | 1992-9-1 |
| DOI | 10.1016/0165-7992(92)90118-2 |
| PII | 0165-7992(92)90118-2 |
| PMID | 1380660 |
| MeSH | Animals Base Sequence Biotransformation Chlorambucil / pharmacology* Chromosome Deletion* Genes, Bacterial / drug effects* Microsomes, Liver / metabolism Molecular Sequence Data Mutagenesis* Mutagenicity Tests Mutagens / pharmacology* Oligodeoxyribonucleotides Plasmids Salmonella typhimurium / drug effects* Salmonella typhimurium / genetics |
| IF | 2.463 |
| リソース情報 | |
| 原核生物(大腸菌) | ME10034 |