論文 - 詳細
| RRC ID | 76857 |
|---|---|
| 著者 | Watanabe M, Ishidate M Jr, Nohmi T. |
| タイトル | A sensitive method for the detection of mutagenic nitroarenes: construction of nitroreductase-overproducing derivatives of Salmonella typhimurium strains TA98 and TA100. |
| ジャーナル | Mutat Res |
| Abstract |
'Classical nitroreductase' is an enzyme involved in the intracellular metabolic activation of mutagenic nitroarenes. The nitroreductase gene of Salmonella typhimurium TA1538 was cloned into pBR322 and the plasmids harboring the gene were introduced into TA98 and TA100. The resulting strains (YG1021 and YG1026) had more than 50 times higher nitrofurazone-reductase activity than TA1538 containing pBR322, and were extremely sensitive to the mutagenic action of 2-nitrofluorene, 1-nitropyrene and 2-nitronaphthalene. These results indicate that the new strains permit the efficient detection of mutagenic nitroarenes. |
| 巻・号 | 216(4) |
| ページ | 211-20 |
| 公開日 | 1989-8-1 |
| DOI | 10.1016/0165-1161(89)90007-1 |
| PII | 0165-1161(89)90007-1 |
| PMID | 2668749 |
| MeSH | Cloning, Molecular Cytosol Genes, Bacterial Mutagens / analysis* Nitro Compounds / analysis* Nitroreductases / biosynthesis* Nitroreductases / genetics Oxidoreductases / biosynthesis* Plasmids Restriction Mapping Salmonella typhimurium / enzymology* Species Specificity Transformation, Bacterial |
| IF | 2.463 |
| リソース情報 | |
| 原核生物(大腸菌) | ME10028 ME10030 |