| RRC ID |
76869
|
| Author |
Hirano Y, Ohno Y, Kubota Y, Fukagawa T, Kihara A, Haraguchi T, Hiraoka Y.
|
| Title |
Ceramide synthase homolog Tlc4 maintains nuclear envelope integrity via its Golgi translocation.
|
| Journal |
J Cell Sci
|
| Abstract |
Maintaining the integrity of the nuclear envelope (NE) is essential for preventing genomic DNA damage. Recent studies have shown that enzymes that catalyze lipid synthesis are involved in NE maintenance, but the underlying mechanism remains unclear. Here, we found that the ceramide synthase (CerS) homolog in the fission yeast Schizosaccharomyces pombe Tlc4 (SPAC17A2.02c) suppressed NE defects in cells lacking the NE proteins Lem2 and Bqt4. Tlc4 possesses a TRAM/LAG1/CLN8 domain that is conserved in CerS proteins and functions through its non-catalytic activity. Tlc4 was localized at the NE and endoplasmic reticulum, similar to CerS proteins, and also showed unique additional localization at the cis- and medial-Golgi cisternae. Growth and mutation analyses revealed that Golgi localization of Tlc4 was tightly linked to its activity of suppressing the defects in the double-deletion mutant of Lem2 and Bqt4. Our results suggest that Lem2 and Bqt4 control the translocation of Tlc4 from the NE to the Golgi, which is necessary for maintaining NE integrity.
|
| Volume |
136(10)
|
| Published |
2023-5-15
|
| DOI |
10.1242/jcs.260923
|
| PII |
310418
|
| PMID |
37078207
|
| MeSH |
Nuclear Envelope / metabolism
Nuclear Proteins / metabolism
Schizosaccharomyces* / genetics
Schizosaccharomyces* / metabolism
Schizosaccharomyces pombe Proteins* / genetics
Schizosaccharomyces pombe Proteins* / metabolism
|
| IF |
4.573
|
| Resource |
| Yeast |
pAL-SK vector |
