Reference - Detail
| RRC ID | 76905 |
|---|---|
| Author | Yamamoto T, Uehara R. |
| Title | Cell shape instability during cytokinesis in tetraploid HCT116 cells. |
| Journal | Biochem Biophys Res Commun |
| Abstract |
Tetraploidy is a hallmark of broad cancer types, but it remains largely unknown which aspects of cellular processes are influenced by tetraploidization in human cells. Here, we found that tetraploid HCT116 cells manifested severe cell shape instability during cytokinesis, unlike their diploid counterparts. The cell shape instability accompanied the formation of protrusive deformation at the cell poles, indicating ectopic contractile activity of the cell cortex. While cytokinesis regulators such as RhoA and anillin correctly accumulated at the equatorial cortex, myosin II was over-accumulated at the cell poles, specifically in tetraploid cells. Suppression of myosin II activity by Y27632 treatment restored smooth cell shape in tetraploids during cytokinesis, indicating dysregulation of myosin II as a primary cause of the cell shape instability in the tetraploid state. Our results demonstrate a new aspect of the dynamic cellular process profoundly affected by tetraploidization in human cells, which provides a clue to molecular mechanisms of tetraploidy-driven pathogenic processes. |
| Volume | 678 |
| Pages | 39-44 |
| Published | 2023-10-20 |
| DOI | 10.1016/j.bbrc.2023.08.038 |
| PII | S0006-291X(23)00984-1 |
| PMID | 37619310 |
| MeSH | Cell Shape Cytokinesis* Cytoskeletal Proteins HCT116 Cells Humans Tetraploidy* |
| IF | 2.985 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | HCT116(RCB2979) |