| RRC ID |
76906
|
| Author |
Mizuno T, Nagano F, Ito Y, Tatsukawa H, Shinoda Y, Takeuchi T, Takahashi K, Tsuboi N, Nagamatsu T, Yamada S, Maruyama S, Hitomi K.
|
| Title |
Novel function of transglutaminase 2 in extracellular histone-induced acute lung injury.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
Extracellular histones induce endothelial damage, resulting in lung haemorrhage; however, the underlying mechanism remains unclear. Factor XIII, as a Ca2+-dependent cross-linking enzyme in blood, mediates fibrin deposition. As another isozyme, transglutaminase 2 (TG2) has a catalytic activity distributing in most tissues. Herein, we investigated whether TG2 promotes fibrin deposition and mediates the adhesion of platelets to ECs in histone-induced acute lung injury (ALI). We evaluated the lung histology and the adhesion of platelets to endothelial cells (ECs) after injecting histones to wild-type (WT) C57BL/6J and TG2 knockout (TG2-/-) mice, and administered a TG2 inhibitor (NC9) to WT mice. Pulmonary haemorrhage was more severe in TG2-/- mice than that in WT mice. The area of fibrin deposition and the proportion of CD41+CD31+ cells were lower in TG2-/- mice than in WT mice. Pre-treatment of NC9 decreased the area of fibrin deposition and the proportion of CD41+CD31+ cells in WT mice. These results suggest that TG2 prevents from pulmonary haemorrhage in ALI by promoting the adhesion of platelets to ECs and the fibrin deposition.
|
| Volume |
678
|
| Pages |
179-185
|
| Published |
2023-10-20
|
| DOI |
10.1016/j.bbrc.2023.08.051
|
| PII |
S0006-291X(23)00997-X
|
| PMID |
37643535
|
| MeSH |
Acute Lung Injury* / chemically induced
Animals
Endothelial Cells*
Fibrin
Histones
Mice
Mice, Inbred C57BL
Protein Glutamine gamma Glutamyltransferase 2
|
| IF |
2.985
|
| Resource |
| Human and Animal Cells |
UV♀2(RCB1994) |
