RRC ID 76913
著者 Matsuda K, Hirayama D, Hino N, Kuno S, Sakaue-Sawano A, Miyawaki A, Matsuda M, Terai K.
タイトル Knockout of all ErbB-family genes delineates their roles in proliferation, survival and migration.
ジャーナル J Cell Sci
Abstract The ErbB-family receptors play pivotal roles in the proliferation, migration and survival of epithelial cells. Because our knowledge on the ErbB-family receptors has been largely obtained by the exogenous application of their ligands, it remains unknown to what extent each of the ErbB members contributes to these outputs. We here knocked out each ErbB gene, various combinations of ErbB genes or all ErbB genes in Madin-Darby canine kidney cells to delineate the contribution of each gene. ERK1 and ERK2 (ERK1/2, also known as MAPK3 and MAPK1, respectively) activation waves during collective cell migration were mediated primarily by ErbB1 and secondarily by the ErbB2 and ErbB3 heterodimer. Either ErbB1 or the ErbB2 and ErbB3 complex was sufficient for the G1/S progression. The saturation cell density was markedly reduced in cells deficient in all ErbB proteins, but not in cells retaining only ErbB2, which cannot bind to ligands. Thus, a ligand-independent ErbB2 activity is sufficient for preventing apoptosis at high cell density. In short, systematic knockout of ErbB-family genes has delineated the roles of each ErbB receptor.
巻・号 136(16)
公開日 2023-8-15
DOI 10.1242/jcs.261199
PII 325936
PMID 37519219
MeSH Animals Cell Proliferation / genetics Dogs Genes, erbB Ligands Phosphorylation Receptor, ErbB-2* / genetics Receptor, ErbB-2* / metabolism Receptor, ErbB-3 / genetics Receptor, ErbB-3 / metabolism Signal Transduction*
IF 4.573
リソース情報
ヒト・動物細胞 MDCK(RCB0995)