論文 - 詳細
| RRC ID | 76916 |
|---|---|
| 著者 | Zhu Z, Becam I, Tovey CA, Elfarkouchi A, Yen EC, Bernard F, Guichet A, Conduit PT. |
| タイトル | Multifaceted modes of γ-tubulin complex recruitment and microtubule nucleation at mitotic centrosomes. |
| ジャーナル | J Cell Biol |
| Abstract |
Microtubule nucleation is mediated by γ-tubulin ring complexes (γ-TuRCs). In most eukaryotes, a GCP4/5/4/6 "core" complex promotes γ-tubulin small complex (γ-TuSC) association to generate cytosolic γ-TuRCs. Unlike γ-TuSCs, however, this core complex is non-essential in various species and absent from budding yeasts. In Drosophila, Spindle defective-2 (Spd-2) and Centrosomin (Cnn) redundantly recruit γ-tubulin complexes to mitotic centrosomes. Here, we show that Spd-2 recruits γ-TuRCs formed via the GCP4/5/4/6 core, but Cnn can recruit γ-TuSCs directly via its well-conserved CM1 domain, similar to its homologs in budding yeast. When centrosomes fail to recruit γ-tubulin complexes, they still nucleate microtubules via the TOG domain protein Mini-spindles (Msps), but these microtubules have different dynamic properties. Our data, therefore, help explain the dispensability of the GCP4/5/4/6 core and highlight the robustness of centrosomes as microtubule organizing centers. They also suggest that the dynamic properties of microtubules are influenced by how they are nucleated. |
| 巻・号 | 222(10) |
| 公開日 | 2023-10-2 |
| DOI | 10.1083/jcb.202212043 |
| PII | 276255 |
| PMID | 37698931 |
| PMC | PMC10497398 |
| MeSH | Animals Centrosome* Cytoskeletal Proteins* / genetics Cytosol Drosophila Drosophila Proteins / genetics Homeodomain Proteins / genetics Microtubule-Organizing Center* Microtubules* / genetics Tubulin* / genetics |
| IF | 8.811 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 52 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | HMJ23752 |