RRC ID 77001
Author Donaldson PD, Navabi ZS, Carter RE, Fausner SML, Ghanbari L, Ebner TJ, Swisher SL, Kodandaramaiah SB.
Title Polymer Skulls With Integrated Transparent Electrode Arrays for Cortex-Wide Opto-Electrophysiological Recordings.
Journal Adv Healthc Mater
Abstract Electrophysiology and optical imaging provide complementary neural sensing capabilities - electrophysiological recordings have high temporal resolution, while optical imaging allows recording of genetically-defined populations at high spatial resolution. Combining these two modalities for simultaneous large-scale, multimodal sensing of neural activity across multiple brain regions can be very powerful. Here, transparent, inkjet-printed electrode arrays with outstanding optical and electrical properties are seamlessly integrated with morphologically conformant transparent polymer skulls. Implanted on transgenic mice expressing the Calcium (Ca2+ ) indicator GCaMP6f in excitatory neurons, these "eSee-Shells" provide a robust opto-electrophysiological interface for over 100 days. eSee-Shells enable simultaneous mesoscale Ca2+ imaging and electrocorticography (ECoG) acquisition from multiple brain regions covering 45 mm2 of cortex under anesthesia and in awake animals. The clarity and transparency of eSee-Shells allow recording single-cell Ca2+ signals directly below the electrodes and interconnects. Simultaneous multimodal measurement of cortical dynamics reveals changes in both ECoG and Ca2+ signals that depend on the behavioral state.
Volume 11(18)
Pages e2200626
Published 2022-9-1
DOI 10.1002/adhm.202200626
PMID 35869830
PMC PMC9573805
MeSH Animals Calcium* Electrodes, Implanted Electrophysiological Phenomena Mice Mice, Transgenic Polymers* Skull
IF 7.367
Mice RBRC09452