| RRC ID |
77010
|
| 著者 |
Ader NR, Chen L, Surovtsev IV, Chadwick WL, Rodriguez EC, King MC, Lusk CP.
|
| タイトル |
An ESCRT grommet cooperates with a diffusion barrier to maintain nuclear integrity.
|
| ジャーナル |
Nat Cell Biol
|
| Abstract |
The molecular mechanisms by which the endosomal sorting complexes required for transport (ESCRT) proteins contribute to the integrity of the nuclear envelope (NE) barrier are not fully defined. We leveraged the single NE hole generated by mitotic extrusion of the Schizosaccharomyces pombe spindle pole body to reveal two modes of ESCRT function executed by distinct complements of ESCRT-III proteins, both dependent on CHMP7/Cmp7. A grommet-like function is required to restrict the NE hole in anaphase B, whereas replacement of Cmp7 by a sealing module ultimately closes the NE in interphase. Without Cmp7, nucleocytoplasmic compartmentalization remains intact despite NE discontinuities of up to 540 nm, suggesting mechanisms to limit diffusion through these holes. We implicate spindle pole body proteins as key components of a diffusion barrier acting with Cmp7 in anaphase B. Thus, NE remodelling mechanisms cooperate with proteinaceous diffusion barriers beyond nuclear pore complexes to maintain the nuclear compartment.
|
| 巻・号 |
25(10)
|
| ページ |
1465-1477
|
| 公開日 |
2023-10-1
|
| DOI |
10.1038/s41556-023-01235-4
|
| PII |
10.1038/s41556-023-01235-4
|
| PMID |
37783794
|
| IF |
20.042
|
| リソース情報 |
| 酵母 |
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