論文 - 詳細
| RRC ID | 77016 |
|---|---|
| 著者 | Oki H, Kaneko MK, Ogasawara S, Tsujimoto Y, Liu X, Sugawara M, Takakubo Y, Takagi M, Kato Y. |
| タイトル | Characterization of Monoclonal Antibody LpMab-7 Recognizing Non-PLAG Domain of Podoplanin. |
| ジャーナル | Monoclon Antib Immunodiagn Immunother |
| Abstract |
Podoplanin (PDPN/Aggrus/T1α), a platelet aggregation-inducing type I transmembrane sialoglycoprotein, is involved in tumor invasion and metastasis. Furthermore, podoplanin expression was reported to be involved in poor prognosis of several cancers. Although many anti-podoplanin monoclonal antibodies (MAbs), such as NZ-1 and D2-40, have been established, those epitopes are limited to platelet aggregation-stimulating (PLAG) domain of podoplanin. In this study, we developed and characterized a novel anti-podoplanin MAb, LpMab-7, that is more sensitive than NZ-1 in immunohistochemistry. We identified the minimum epitope of LpMab-7 as Arg79-Leu83 of human podoplanin, which is different from PLAG domain, using ELISA, Western blot analysis, and flow cytometry. Because LpMab-7 has high sensitivity against podoplanin, LpMab-7 is expected to be useful for molecular targeting therapy for podoplanin-expressing cancers. |
| 巻・号 | 34(3) |
| ページ | 174-80 |
| 公開日 | 2015-6-1 |
| DOI | 10.1089/mab.2014.0090 |
| PMID | 26090595 |
| MeSH | Antibodies, Monoclonal / immunology* Antibody Specificity Blotting, Western Bone Neoplasms / diagnosis* Bone Neoplasms / immunology Epitopes / immunology* Flow Cytometry Humans Immunoenzyme Techniques Membrane Glycoproteins / immunology* Osteosarcoma / diagnosis* Osteosarcoma / immunology Platelet Aggregation / immunology* Tumor Cells, Cultured |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | OST(RCB0454) |