Reference - Detail
| RRC ID | 77021 |
|---|---|
| Author | Cermak N, Olcum S, Delgado FF, Wasserman SC, Payer KR, A Murakami M, Knudsen SM, Kimmerling RJ, Stevens MM, Kikuchi Y, Sandikci A, Ogawa M, Agache V, Baléras F, Weinstock DM, Manalis SR. |
| Title | High-throughput measurement of single-cell growth rates using serial microfluidic mass sensor arrays. |
| Journal | Nat Biotechnol |
| Abstract |
Methods to rapidly assess cell growth would be useful for many applications, including drug susceptibility testing, but current technologies have limited sensitivity or throughput. Here we present an approach to precisely and rapidly measure growth rates of many individual cells simultaneously. We flow cells in suspension through a microfluidic channel with 10-12 resonant mass sensors distributed along its length, weighing each cell repeatedly over the 4-20 min it spends in the channel. Because multiple cells traverse the channel at the same time, we obtain growth rates for >60 cells/h with a resolution of 0.2 pg/h for mammalian cells and 0.02 pg/h for bacteria. We measure the growth of single lymphocytic cells, mouse and human T cells, primary human leukemia cells, yeast, Escherichia coli and Enterococcus faecalis. Our system reveals subpopulations of cells with divergent growth kinetics and enables assessment of cellular responses to antibiotics and antimicrobial peptides within minutes. |
| Volume | 34(10) |
| Pages | 1052-1059 |
| Published | 2016-10-1 |
| DOI | 10.1038/nbt.3666 |
| PII | nbt.3666 |
| PMID | 27598230 |
| PMC | PMC5064867 |
| MeSH | Cell Proliferation / drug effects* Cell Proliferation / physiology* Drug Evaluation, Preclinical / instrumentation* Drug Evaluation, Preclinical / methods Equipment Design Equipment Failure Analysis High-Throughput Screening Assays / instrumentation* High-Throughput Screening Assays / methods Lab-On-A-Chip Devices* Micro-Electrical-Mechanical Systems / instrumentation* Micro-Electrical-Mechanical Systems / methods Reproducibility of Results Sensitivity and Specificity Transducers |
| IF | 36.553 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 97 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | Ba/F3 |