RRC ID 77035
Author Viriyaadhammaa N, Saiai A, Neimkhum W, Nirachonkul W, Chaiyana W, Chiampanichayakul S, Tima S, Usuki T, Duangmano S, Anuchapreeda S.
Title Cytotoxic and Antiproliferative Effects of Diarylheptanoids Isolated from Curcuma comosa Rhizomes on Leukaemic Cells.
Journal Molecules
Abstract Curcuma comosa belongs to the Zingiberaceae family. In this study, two natural compounds were isolated from C. comosa, and their structures were determined using nuclear magnetic resonance. The isolated compounds were identified as 7-(3,4-dihydroxyphenyl)-5-hydroxy-1-phenyl-(1E)-1-heptene (1) and trans-1,7-diphenyl-5-hydroxy-1-heptene (2). Compound 1 showed the strongest cytotoxicity effect against HL-60 cells, while its antioxidant and anti-inflammatory properties were stronger than those of compound 2. Compound 1 proved to be a potent antioxidant, compared to ascorbic acid. Neither compounds had any effect on red blood cell haemolysis. Furthermore, compound 1 significantly decreased Wilms' tumour 1 protein expression and cell proliferation in KG-1a cells. Compound 1 decreased the WT1 protein levels in a time- and dose- dependent manner. Compound 1 suppressed cell cycle at the S phase. In conclusion, compound 1 has a promising chemotherapeutic potential against leukaemia.
Volume 25(22)
Published 2020-11-23
DOI 10.3390/molecules25225476
PII molecules25225476
PMID 33238470
PMC PMC7700379
MeSH Animals Anti-Inflammatory Agents / chemistry Anti-Inflammatory Agents / isolation & purification Anti-Inflammatory Agents / pharmacology Antineoplastic Agents / chemistry* Antineoplastic Agents / isolation & purification Antineoplastic Agents / pharmacology* Antioxidants / chemistry Antioxidants / isolation & purification Antioxidants / pharmacology Cell Cycle / drug effects Cell Line, Tumor Cell Proliferation / drug effects Chromatography / methods Curcuma / chemistry* Diarylheptanoids / chemistry* Diarylheptanoids / isolation & purification Diarylheptanoids / pharmacology* Dose-Response Relationship, Drug Flow Cytometry Gene Expression Hemolysis Humans Leukemia / genetics Leukemia / metabolism Leukocytes, Mononuclear / drug effects Leukocytes, Mononuclear / metabolism Mice Molecular Structure Plant Extracts / chemistry* Plant Extracts / isolation & purification Plant Extracts / pharmacology* RAW 264.7 Cells Rhizome / chemistry* WT1 Proteins / genetics WT1 Proteins / metabolism
IF 3.267
Human and Animal Cells K562(RCB1897)