Reference - Detail
| RRC ID | 77053 |
|---|---|
| Author | Yoshioka I, Mori Y, Fahal AH, Siddig EE, Kaneko S, Yaguchi T. |
| Title | Specific and sensitive loop-mediated isothermal amplification (LAMP) method for Madurella strains, eumycetoma filamentous fungi causative agent. |
| Journal | PLoS Negl Trop Dis |
| Abstract |
BACKGROUND:Filamentous fungi of the genus Madurella are the primary causative agents of mycetoma, a disease observed in tropical and subtropical regions. Since early diagnostics based on a morphological approach are difficult and have many shortcomings, a molecular diagnostic method suitable for rural settings is required. In this study, we developed the loop-mediated isothermal amplification (LAMP) method to present a foundational technique of the diagnosis of Madurella spp. (M. mycetomatis, M. pseudomycetomatis, M. tropicana, and M. fahalii), the common causative organisms of eumycetoma. PRINCIPAL FINDINGS:We successfully designed a primer pair targeting the rDNAs of three Madurella spp. excluding M. fahalii, and detected up to 100 fg of genomic DNA extracted from isolates of M. mycetomatis and 1 pg of M. pseudomycetomatis and M. tropicana, within one hour. Second, a primer pair specific to M. mycetomatis, the most common causative species, or M. fahalii, a drug-resistant species, was constructed, and the detection limit of both primer pairs was 1 pg. The designed primers accurately distinguished 16 strains of the genus Madurella from various fungal species known to cause mycetomas. CONCLUSION:In summary, we established the first model of a LAMP detection method that rapidly and sensitively detects and identifies Madurella isolates for clinical diagnostics. Moreover, the combined designed primer sets could identify mycetoma-causing strains simultaneously. |
| Volume | 17(9) |
| Pages | e0011644 |
| Published | 2023-9-1 |
| DOI | 10.1371/journal.pntd.0011644 |
| PII | PNTD-D-23-00841 |
| PMID | 37721946 |
| PMC | PMC10538720 |
| MeSH | Madurella* Mycetoma* / diagnosis Mycetoma* / microbiology Nucleic Acid Amplification Techniques / methods |
| IF | 3.885 |
| Resource | |
| Pathogenic eukaryotic microorganisms | Madurella mycetomatis IFM 46458, Madurella pseudomycetomatis IFM 46460 |