RRC ID 77058
Author Jian Zhu, Jiafei Cao, Tingying Li, Qi Liu, Yingqian Kang, Zhenghong Chen, Wieland Meyer, Yuzuru Mikami, Mei Long
Title The Predominance of a Specific Genotype of Cryptococcus neoformans var. Grubii in China and Japan
Journal Computational and Mathematical Methods in Medicine
Abstract Background. The extensive deployment of molecular genotyping methods is the top reliable keyword to characterize the population genetic structure of C. neoformans in the past decade. However, studies involving genotypic analysis of C. neoformans var. grubii from China and Japan are limited. Objectives. We address this challenge to determine the genotype distribution of C. neoformans var. grubii strains from China and Japan. Methods. Genotypic analysis of 52 C. neoformans var. grubii isolates was performed using multilocus microsatellite typing (MLMT) based on the sequence analysis of 3 functional genes. In order to place the herein-studied strains into the global picture, 22 strains randomly selected from the 52 strains studied by MLMT were also analyzed by restriction fragment length polymorphism analysis of the orotidine monophosphate pyrophosphorylase gene (URA5-RFLP), M13 PCR-fingerprinting, and multilocus sequence typing (MLST). Results. MLMT classified 46 (88.5%) of the 52 strains as genotype MLMT-17. The high prevalence of the MLMT-17 type was observed for environmental and clinical isolates from China and Japan. URA5-RFLP analysis, M13 PCR-fingerprinting, and MLST showed that most of these belong to the VNI/ST5 (M5) genotype. Conclusions. Our study suggests the predominance of a specific genotype of C. neoformans var. grubii in China and Japan.
Volume 2022
Pages 1-10
Published 2022-8-5
DOI 10.1155/2022/8162096
PMID 36035289
PMC PMC9410922
MeSH Cryptococcosis* Cryptococcus neoformans* Genotype Humans Japan Multilocus Sequence Typing Mycological Typing Techniques
Pathogenic eukaryotic microorganisms Cryptococcus neoformans IFM 45772