Reference - Detail
| RRC ID | 77161 |
|---|---|
| Author | Yu J, Leung WK, Lee TL, Tse PC, To KF, Sung JJ. |
| Title | Promoter hypermethylation of cyclooxygenase-2 in gastric carcinoma. |
| Journal | Int J Oncol |
| Abstract |
Overexpression of cyclooxygenase-2 (COX-2) is associated with loss of apoptosis, enhancement of proliferation and tumorigenesis. The role of promoter methylation in the transcriptional silencing of cox-2 gene in human gastric cancer is less determined. We investigated 5 gastric cancer cell lines and 58 primary gastric carcinomas for the presence of promoter hypermethylation in cox-2 gene. Combined methylation-specific polymerase chain reaction analysis and bisulfite sequencing analysis revealed that the cox-2 promoter was methylated in 2 of the gastric cancer cell lines. Treatment with 5-aza-2'-deoxycytidine, a DNA methyltransferase inhibitor, induced COX-2 expression in the methylated gastric cancer cell line. Among the 58 primary gastric cancers, hypermethylation was detected in 25 (43.1%) cases. However, none of the normal gastric tissues showed methylation in cox-2. Promoter hypermethylation was associated with loss of protein expression as determined by immunostaining (p=0.005). Our results indicate that hypermethylation of the CpG island in the cox-2 gene is a major mechanism that mediates transcriptional silencing in a subset of gastric cancers. Thus, gastric cancers with methylation in cox-2 may not be good candidates for treatment with specific COX-2 inhibitors. |
| Volume | 22(5) |
| Pages | 1025-31 |
| Published | 2003-5-1 |
| PMID | 12684668 |
| MeSH | Adenocarcinoma / enzymology Adenocarcinoma / genetics Adenocarcinoma / pathology Adenocarcinoma / surgery Adult Aged Base Sequence Cyclooxygenase 2 DNA Methylation* DNA Primers Female Gastrectomy Gene Expression Regulation, Neoplastic* Humans Immunohistochemistry Isoenzymes / genetics* Male Membrane Proteins Middle Aged Polymerase Chain Reaction Promoter Regions, Genetic* Prostaglandin-Endoperoxide Synthases / genetics* RNA, Messenger / genetics Reverse Transcriptase Polymerase Chain Reaction Stomach Neoplasms / enzymology Stomach Neoplasms / genetics* Stomach Neoplasms / pathology Stomach Neoplasms / surgery Transcription, Genetic Tumor Cells, Cultured |
| IF | 1.95 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | MKN28(RCB1000) MKN45(RCB1001) |