論文 - 詳細
| RRC ID | 77173 |
|---|---|
| 著者 | Sasakura Y, Horie T. |
| タイトル | Improved Genome Editing in the Ascidian Ciona with CRISPR/Cas9 and TALEN. |
| ジャーナル | Methods Mol Biol |
| Abstract |
The ascidian Ciona intestinalis type A (or Ciona robusta) is an important organism for elucidating the mechanisms that make the chordate body plan. CRISPR/Cas9 and TAL effector nuclease (TALEN) are widely used to quickly address genetic functions in Ciona. Our previously reported method of CRISPR/Cas9-mediated mutagenesis in this animal has inferior mutation rates compared to those of TALENs. We here describe an updated way to effectively mutate genes with CRISPR/Cas9 in Ciona. Although the construction of TALENs is much more laborious than that of CRISPR/Cas9, this technique is useful for tissue-specific knockouts that are not easy even by the optimized CRISPR/Cas9 method. |
| 巻・号 | 2637 |
| ページ | 375-388 |
| 公開日 | 2023-1-1 |
| DOI | 10.1007/978-1-0716-3016-7_28 |
| PMID | 36773161 |
| MeSH | Animals CRISPR-Cas Systems / genetics Ciona* / metabolism Ciona intestinalis* / genetics Ciona intestinalis* / metabolism Gene Editing / methods Gene Knockout Techniques Transcription Activator-Like Effector Nucleases / genetics Transcription Activator-Like Effector Nucleases / metabolism Transcription Activator-Like Effectors / genetics |
| リソース情報 | |
| カタユウレイボヤ・(ニッポンウミシダ) | Wild C. int pHTB-TALEN-NG::2A::mCherry pHTB-TALEN-NI::2A::mCherry pHTB-TALEN-NN::2A::mCherry pHTB-TALEN-HD::2A::mCherry pEF1a>TALEN-NG::2A::mCherry pEF1a>TALEN-NI::2A::mCherry pEF1a>TALEN-NN::2A::mCherry pEF1a>TALEN-HD::2A::mCherry |